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NACRES:
NA.25
UNSPSC Code:
41105319
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Molecular Biology
description
Saline-Sodium Citrate buffer, made with ultrapure water
sterility
0.2 μm filtered
form
solution
Quality Level
color
colorless
pH
6.9 —7.1
application(s)
diagnostic assay manufacturing
foreign activity
DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected
storage temp.
room temp
Catégories apparentées
General description
SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.
Application
SSC Buffer 20× Concentrate has been used
- as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence
- as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNA
- as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon
Features and Benefits
- Convenient ready-to-use liquid
- Multi-purpose use in hybridizations
Preparation Note
SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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