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Merck

SAB1305538

MONOCLONAL ANTI-HIS TAG antibody produced in mouse

clone 6AT18, IgG fraction of antiserum, buffered aqueous solution

Synonyme(s) :

Mouse Anti-Hexa Histidine tag

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A propos de cet article

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
6AT18, monoclonal
Technique(s):
western blot: 1:1000
Application:
western blot
Uniprot accession no.:
Citations:
39
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Nom du produit

MONOCLONAL ANTI-HIS TAG antibody produced in mouse, clone 6AT18, IgG fraction of antiserum, buffered aqueous solution

biological source

mouse

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

6AT18, monoclonal

form

buffered aqueous solution

technique(s)

western blot: 1:1000

isotype

IgG1

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent′s anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.

Immunogen

Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.

Physical form

Supplied in PBS with 0.09% (W/V) sodium azide

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Classe de stockage

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Jialing Bao et al.
Frontiers in cellular and infection microbiology, 11, 694957-694957 (2021-06-08)
Microsporidia are a group of spore-forming, fungus-related pathogens that can infect both invertebrates and vertebrates including humans. The primary infection site is usually digestive tract, but systemic infections occur as well and cause damages to organs such as lung, brain
Jenifer E Shattuck et al.
Nature communications, 10(1), 3614-3614 (2019-08-11)
Stress granules are membraneless protein- and mRNA-rich organelles that form in response to perturbations in environmental conditions. Stress granule formation is reversible, and persistent stress granules have been implicated in a variety of neurodegenerative disorders, including amyotrophic lateral sclerosis. However
Pooja Yadav et al.
Retrovirology, 16(1), 12-12 (2019-05-01)
The different interactions between viral proteins and cellular host proteins are required for efficient replication of HIV-1. Various reports implicated host cellular proteins as a key factor that either interact directly with HIV-1 integrase (IN) or get involved in the
Anna-Lena Kolb et al.
Methods in molecular biology (Clifton, N.J.), 1813, 125-148 (2018-08-12)
The amoeba Dictyostelium discoideum is a single-cell organism that can undergo a simple developmental program, making it an excellent model to study the molecular mechanisms of cell motility, signal transduction, and cell-type differentiation. A variety of human genes that are
Fumin Shi et al.
Cell reports, 37(3), 109834-109834 (2021-10-24)
WNTs play key roles in development and disease, signaling through Frizzled (FZD) seven-pass transmembrane receptors and numerous co-receptors including ROR and RYK family receptor tyrosine kinases (RTKs). We describe crystal structures and WNT-binding characteristics of extracellular regions from the Drosophila

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