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Merck

SAB4200194

Anti-Pontin antibody, Mouse monoclonal

clone 5G3-11, purified from hybridoma cell culture

Synonyme(s) :

Anti-49 kDa TATA box-binding protein-interacting protein, Anti-49 kDa TBP-interacting protein, Anti-54 kDa erythrocyte cytosolic protein, Anti-ECP-54, Anti-INO80 complex subunit H, Anti-NMP 238, Anti-Pontin52, Anti-RUVBL1, Anti-RVB1, Anti-RuvB (e coli homolog)-like 1, Anti-RuvB-like 1, Anti-TAP54-alpha, Anti-TIH1, Anti-TIP49, Anti-TIP49A, Anti-TIP60-associated protein 54-alpha, Anti-nuclear matrix protein 238

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A propos de cet article

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
5G3-11, monoclonal
Application:
IF, IHC, IP, WB
Citations:
16

Principaux documents

Voir toute la documentation
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biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

5G3-11, monoclonal

form

buffered aqueous solution

mol wt

~50 kDa

species reactivity

canine, human, monkey

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect immunofluorescence: suitable, western blot: 1:1,000-1:2,000 using whole extracts of human HEK-293T or MCF-7 cells

isotype

IgG

UniProt accession no.

Q9Y265

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... RUVBL1(8607)

General description

Monoclonal Anti-Pontin (mouse IgG1 isotype) is derived from the hybridoma 5G3-11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a human pontin fusion protein. Pontin and reptin are two related members of the AAA+ (ATPases associated with diverse cellular activities) family of ATPases. Pontin and reptin share some homology to the bacterial RuvB gene.

Immunogen

human Pontin fusion protein

Application

Monoclonal Anti-Pontin antibody produced in mouse has been used in:
  • western blotting
  • immunoprecipitation
  • ChIP-sequencing
  • immunohistochemistry
  • immunofluorescence
  • RNA immunoprecipitation

Biochem/physiol Actions

Pontin and Reptin are associated with several chromatin-remodeling complexes and are involved in multiple biological processes including chromatin remodeling, DNA damage repair, transcriptional regulation, telomerase activity, apoptosis and cancer metastasis. Both proteins are also involved in cellular transformation by beta-catenin and c-myc through their chromatin remodeling function.

Physical form

Solution in 0.01 M phosphate buffered saline, 7.4 pH, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Classe de stockage

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000506440
0000506440
0000131200
0000127028
048M4777V

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

The Emergence of the Conserved AAA+ ATPases Pontin and Reptin on the Signaling Landscape
Rosenbaum J, et al.
Science Signaling, 6(266), mr1-mr1 (2013)
Gabrijela Dumbovic et al.
Nucleic acids research, 46(11), 5504-5524 (2018-06-19)
Primate-specific NBL2 macrosatellite is hypomethylated in several types of tumors, yet the consequences of this DNA hypomethylation remain unknown. We show that NBL2 conserved repeats are close to the centromeres of most acrocentric chromosomes. NBL2 associates with the perinucleolar region
A novel long non-coding RNA from NBL2 pericentromeric macrosatellite forms a perinucleolar aggregate structure in colon cancer
Dumbovic G, et al.
Nucleic Acids Research, 46(11), 5504- 5524 (2018)
Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells
Boo K, et al.
Nature Communications, 6810-6810 (2015)
Recruitment of Pontin/Reptin by E2f1 amplifies E2f transcriptional response during cancer progression
Tarangelo A, et al.
Nature Communications, 6(6), 10028-10028 (2015)
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