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Merck

SAB4200544

Anti-Synaptophysin antibody, Mouse monoclonal

clone SVP-38, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-MRXSYP, Monoclonal Anti-SYP, Monoclonal Anti-Synaptophysin1, Monoclonal Anti-Synaptoporin, Monoclonal Anti-Syp1, Monoclonal Anti-p38

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A propos de cet article

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
SVP-38, monoclonal
Application:
IF, IHC, WB
Citations:
25

Principaux documents

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biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SVP-38, monoclonal

form

buffered aqueous solution

mol wt

antigen 38 kDa

species reactivity

rat, human

concentration

~1.0 mg/mL

technique(s)

immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded rat cerebellum., indirect immunofluorescence: 10-20 μg/mL using B35 cells., western blot: 1-2 μg/mL using newborn rat brain extracts.

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... SYP(6855)

General description

Anti-Synaptophysin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma SVP-38 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synaptosome preparation from rat retina. Synaptophysin is a glycoprotein containing four transmembrane domains with both N and C termini on the cytoplasm face.
Synaptophysin (SYP) is encoded by the gene mapped to human chromosome Xp11.23-p11.22. The encoded protein has a molecular mass of 38kDa and is exclusively localized to the membrane of presynaptic vesicles.

Immunogen

synaptosome preparation from rat retina. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.

Application

Anti-Synaptophysin antibody, Mouse monoclonal has been used in following immunochemical techniques:
  • Immunoblotting
  • Immunohistochemistry
  • Immunofluorescence

Biochem/physiol Actions

Anti-Synaptophysin antibody, Mouse monoclonal recognizes rat, human, guinea-pig, and pig synaptophysin
Synaptophysin (SYP) facilitates neurotransmitter release and synaptic plasticity. It is also implicated in synaptic vesicle formation and exocytosis. Mutation in the gene increases the risk of susceptibility to schizophrenia. It is widely used as a potential marker to study the circulation of synapses in the brain and to discover the basic features of the life cycle of synaptic vesicles (SVs).
Synaptophysin has also been implicated in the recycling of synaptic vesicles by associating with dynamin I, a GTPase required for endocytosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Classe de stockage

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
059M4772V
059M4772V
127M4754V
066M4774V
085M4856V

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Association between SYP with attention-deficit/hyperactivity disorder in Chinese Han subjects: differences among subtypes and genders.
Liu L
Psychiatry Research, 210, 308-314 (2013)
Regulation of synaptophysin degradation by mammalian homologues of seven in absentia
Wheeler TC, et al.
The Journal of Biological Chemistry, 277(12), 10273-10282 (2002)
Neuronal connections of direct and indirect pathways for stable value memory in caudal basal ganglia
Amita H, et al.
The European Journal of Neuroscience, 49(5), 712-725 (2019)
Mikihito Shibata et al.
Nature, 598(7881), 489-494 (2021-10-03)
The similarities and differences between nervous systems of various species result from developmental constraints and specific adaptations1-4. Comparative analyses of the prefrontal cortex (PFC), a cerebral cortex region involved in higher-order cognition and complex social behaviours, have identified true and
Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles
Wiedenmann B and Franke WW
Cell, 41, 1017-1028 (1985)
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