SRP3211
IL-4 from mouse
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonyme(s) :
B cell stimulating factor (BSF-1), BCDF, BCGF
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A propos de cet article
NACRES:
NA.32
UNSPSC Code:
12352202
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Laissez-nous vous aiderNom du produit
IL-4 from mouse, Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
biological source
mouse
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
≤2.0 ng/mL ED50
mol wt
13.7 kDa
packaging
pkg of 20 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... IL4(16189)
Biochem/physiol Actions
It regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 (T helper) cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 (immunoglobulin G1) and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. IL-4 helps in long-term recovery after cerebral ischemia by controlling the activities of microglia/macrophage. In mice, absence of IL-4 is associated with a state anxiety-like behavior.
General description
IL-4 (interleukin 4) is a pleiotropic cytokine. Produced by mast cells, T cells and bone marrow stromal cells. Recombinant murine IL-4 is a 13.7kDa globular protein containing 120 amino acid residues.
Other Notes
MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S
Physical form
Lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Mast cell production and response to IL-4 and IL-13.
McLeod JJ
Cytokine, 75, 57-61 (2015)
Chih-Wei Ko et al.
Bio-protocol, 8(17), e3003-e3003 (2018-09-05)
Using gas chromatography mass spectrometry (GC-MS) to analyze the citric acid cycle (CAC) and related intermediates (such as glutamate, glutamine, GABA, and aspartate) is an analytical approach to identify unexpected correlations between apparently related and unrelated pathways of energy metabolism.
Zhiqiang Huang et al.
STAR protocols, 3(2), 101338-101338 (2022-05-03)
Chromosome conformation capture combined with high-throughput sequencing (4C-seq) is a powerful tool to map genomic DNA regions that communicate with a specific locus of interest such as functional single-nucleotide polymorphism (SNPs)-containing regions. This protocol describes detailed steps to perform 4C-seq
Saeko Nakajima et al.
The Journal of investigative dermatology, 134(8), 2122-2130 (2014-02-01)
Atopic dermatitis (AD) is generally regarded as a type 2 helper T (Th2)-mediated inflammatory skin disease. Although the number of IL-17A-producing cells is increased in the peripheral blood and in acute skin lesion of AD patients, the role of IL-17A
Interleukin (IL)-6 directs the differentiation of IL-4-producing CD4+ T cells.
Rincon M
The Journal of Experimental Medicine, 185, 461-469 (1997)
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