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Merck

T3526

Anti-TUBA1B (alpha-tubulin ubiquitous) Antibody

rabbit polyclonal

Synonyme(s) :

Anti-K-ALPHA-1

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00145921
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, WB
Citations:
62

Principaux documents

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Nom du produit

Anti-Tubulin antibody produced in rabbit, whole antiserum

biological source

rabbit

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

chicken, mouse

technique(s)

indirect immunofluorescence: 1:80 using cultured fibroblasts, western blot: 1:200 using chicken gizzard extract or mouse brain extract

UniProt accession no.

P68363

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

mouse ... Tuba1b(22143), Tubb2a(22151), Tubb2b(73710), Tubb2c(227613)

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Catégories apparentées

General description

Microtubules are composed of tubulin and several additional proteins designated microtubule associated proteins (MAPs). Tubulin is a heterodimer which consists of an α-chain and a β-chain, both of which have a molecular weight of approximately 55,000. Microtubules form an elaborate cytoplasmic network during interphase and are reorganized into the mitotic spindle during cell division.
Tubulin is an extremely conserved and negatively charged protein. It is expressed in all eukaryotic cells, but mainly in brains.

Immunogen

microtubule proteins from brains of 13 day old chicken embryos

Application

Anti-Tubulin antibody produced in rabbit has been used in immunoblotting and indirect immunofluorescence.

Biochem/physiol Actions

αβ-tubulin heterodimers helps in formation of microtubules. Tubulin dimers exhibits GTPase activity, assembly and disassembly properties. It facilitates the construction of the flagellar apparatus.
The product stains tubulin in cultured chicken fibroblasts or 3T3 mouse fibroblasts. Additional bands may be stained in immunoblotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Classe de stockage

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000508184
0000508184
0000359483
0000359483
0000237218

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Microtubule proteins and their post-translational forms in the cerebrospinal fluid of patients with paraparesis associated with HTLV-I infection and in SH-SY5Y cells: an in vitro model of HTLV-I-induced disease
Maldonado H, et al.
Biological Research, 41(3), 239-259 (2008)
Hong Zhu et al.
Experimental and therapeutic medicine, 15(3), 2643-2648 (2018-02-20)
The aim of the present study was to investigate the mechanism of action by which naringin reverses the resistance of ovarian cancer cells to cisplatin. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and western blotting assays were used to detect
Tumor necrosis factor-related apoptosis-inducing ligand promotes migration of human bone marrow multipotent stromal cells
Secchiero P, et al.
Stem Cells, 26(11), 2955-2963 (2008)
Jorge F Giani et al.
Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society, 22(6), 224-233 (2012-09-06)
Growth hormone (GH) resistance leads to enhanced insulin sensitivity, decreased systolic blood pressure and increased lifespan. The aim of this study was to determine if there is a shift in the balance of the renin-angiotensin system (RAS) towards the ACE2/Ang-(1-7)/Mas
Isolating tubulin from nonneural sources
Methods in Cell Biology, 95, 17-32 (2010)
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Contenu apparenté

Data Sheet - T3526

Instructions

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