T7539
Tumor Necrosis Factor-α from mouse
≥95%, recombinant, expressed in E. coli, powder, suitable for cell culture
Synonyme(s) :
mTNF-α, TNF-α
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A propos de cet article
Biological source:
mouse
Recombinant:
expressed in E. coli
Assay:
≥95%
Form:
powder
Mol wt:
17 kDa
Impurities:
≤1 EU/μg protein endotoxin
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Laissez-nous vous aiderNom du produit
Tumor Necrosis Factor-α from mouse, TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
biological source
mouse
recombinant
expressed in E. coli
assay
≥95%
form
powder
potency
0.01-0.5 ng/mL EC50
mol wt
17 kDa
packaging
pkg of 10 μg, pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg protein endotoxin
color
white
solubility
water: soluble
UniProt accession no.
storage temp.
−20°C
Quality Level
Application
Tumor Necrosis Factor-α from mouse, a potent proinflammatory cytokine has been used:
- to activate MS1 endothelium cells
- to induce lipolysis in adipocytes
- to study its effect on liganded and unliganded glucocorticoid receptor (GR) in interleukin (IL)-6 and IL-8 gene regulation
Biochem/physiol Actions
Tumor necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is glycosylated.
Tumor necrosis factor-α, also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is cytotoxic for many transformed cells, but in normal diploid cells, it stimulates proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and induces production of several other cytokines.
Physical form
Lyophilized from 10 mM sodium phosphate, pH 7.5
Other Notes
One unit is the amount required to induce half-maximal cytolysis of L929 cells.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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SDF-1--CXCR4 differentially regulates autoimmune diabetogenic T cell adhesion through ROBO1--SLIT2 interactions in mice
Glawe JD, et al.
Diabetologia, 56(10), 2222-2230 (2013)
Relative contribution of adipose triglyceride lipase and hormone-sensitive lipase to tumor necrosis factor-$\alpha$ (TNF-$\alpha$)-induced lipolysis in adipocytes
Yang X, et al.
The Journal of Biological Chemistry, 286(47), 40477-40485 (2011)
Glucocorticoid-independent repression of tumor necrosis factor (TNF) $\alpha$-stimulated interleukin (IL)-6 expression by the glucocorticoid receptor a potential mechanism for protection against an excessive inflammatory response
Verhoog NJD, et al.
The Journal of Biological Chemistry, 286(22), 19297-19310 (2011)
Fen Li et al.
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 40(9), 945-954 (2015-09-27)
To investigate the association of TNF-α-308 gene polymorphism with the risk of RA in Hunan's Han Chinese population. A total of 112 patients with RA from Han Chinese population in Hunan were recruited, along with 129 healthy controls. TNF-α-308 (rs1800629)
Fabrícia Lima Fontes et al.
BMC medical genetics, 16, 70-70 (2015-09-01)
Bacterial meningitis (BM) is an infectious disease that results in high mortality and morbidity. Despite efficacious antibiotic therapy, neurological sequelae are often observed in patients after disease. Currently, the main challenge in BM treatment is to develop adjuvant therapies that
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