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Merck

T8778

Trehalase from porcine kidney

buffered aqueous glycerol solution, ≥1.0 units/mg protein

Synonyme(s) :

α,α-Trehalose glucohydrolase

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A propos de cet article

Numéro CAS:
9025-52-9
Numéro CE :
3.2.1.28 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
MFCD00132462
Specific activity:
≥1.0 units/mg protein
Concentration:
0.5-10.0 mg/mL protein (BCA)

Principaux documents

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form

buffered aqueous glycerol solution

specific activity

≥1.0 units/mg protein

concentration

0.5-10.0 mg/mL protein (BCA)

storage temp.

−20°C

Quality Level

200

Catégories apparentées

General description

Trehalase is a glycosidase that occurs in many forms and is found in all tissues. It belongs to the glycoside hydrolase 37 (GH37) family of the carbohydrate-active enzyme (CAZy) classification.
Trehalose is the main haemolymph sugar in most insects and is potentially a prime target for an invading pathogenic fungus.

Application

Trehalase has been used:
  • in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves
  • in a study to investigate growth arrest by trehalose-6-phosphate
  • to quantify the intracellular trehalose in Lactobacillus casei cells

Biochem/physiol Actions

Trehalase catalyzes the hydrolysis of trehalose to produce two glucose units. It mediates flight metabolism, molting induced chitin production and tolerance towards cold temperature.

Physical form

Solution in 50% glycerol containing 1% Triton™ X-100 and 25 mM potassium phosphate, pH 6.5

Other Notes

One unit will convert 1.0 μmole of trehalose to 2.0 μmoles of glucose per min at pH 5.7 at 37 °C (liberated glucose determined at pH 7.5).

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Classe de stockage

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000280313
0000352066
0000352227
0000280313
0000352066

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How is Product T8778, Trehalase from porcine kidney prepared?

    The actual method of preparation utilized by Sigma for Product T8778, Trehalase is confidential, but does include ion exchange and size exclusion chromatography. A method of preparation for Trehalase has been described in Archives of Biochemistry and Biophysics, 255, 168 - 175 (1987).

  6. What is the molecular weight of Product T8778, Trehalase from porcine kidney?

    The molecular weight of Product T8778, Trehalase from porcine kidney has an estimated molecular weight of 80,000 daltons upon SDS-PAGE, as described by Yoshitaka Yoneyama in Archives of Biochemistry and Biophysics, 255, 168 - 175 (1987). This molecular weight is similar to that reported for mouse Trehalase which has an estimated molecular weight by gel filtration on Sephadex G-200 of 73,000 daltons, as described in Anais Da Academia Brasileira De Ciencias, 51, 151 - 158 (1979). 

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