50867-U
Ascentis® Express 90 Å F5 (2 μm) HPLC Columns
L × I.D. 15 cm × 2.1 mm UHPLC Column
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A propos de cet article
UNSPSC Code:
41115700
NACRES:
SB.52
L × i.d.:
15 cm × 2.1 mm
Particle size:
2 μm
Matrix active group:
PFP (pentafluorophenyl) phase
Pore size:
90 Å pore size
Matrix:
Fused-Core particle platform, superficially porous particle
Service technique
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Laissez-nous vous aiderNom du produit
Ascentis® Express F5, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 15 cm × 2.1 mm
material
stainless steel column
Quality Level
agency
suitable for USP L43
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
1000 bar max. pressure (14500 psi), 60 °C temp. range
technique(s)
LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable
L × I.D.
15 cm × 2.1 mm
surface area
120 m2/g
impurities
<5 ppm metals
matrix
Fused-Core particle platform, superficially porous particle
matrix active group
PFP (pentafluorophenyl) phase
particle size
2 μm
pore size
90 Å pore size
operating pH
2-8
application(s)
food and beverages
separation technique
reversed phase
General description
Ascentis® Express 2.0 μm F5 is a high-speed, UHPLC column based on a Fused-Core® particle design. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4 μm thick porous shell and the small overall particle size of 2 μm. The bonded, endcapped, dimethylpentafluorophenylpropyl stationary phase of Ascentis® Express 2.0 μm F5 provides a stable, reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Having polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis® Express 2.0 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
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Contenu apparenté
Ascentis® Express 2.0 μm F5 Column Care & Use Sheet
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On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar