53345-U
Ascentis® Express Phenyl-Hexyl (2.7 μm) HPLC Column
L × I.D. 10 cm × 3 mm, pk of 1 ea
Synonyme(s) :
Core-shell (SPP) Fused Core Phenyl HPLC column
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A propos de cet article
UNSPSC Code:
41115700
NACRES:
SB.52
eCl@ss:
32110501
L × i.d.:
10 cm × 3 mm
Particle size:
2.7 μm
Matrix active group:
phenylhexyl phase
Pore size:
90 Å
Matrix:
Fused-Core particle platform, superficially porous particle
Service technique
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stainless steel column
Quality Level
agency
suitable for USP L11
product line
Ascentis®
feature
endcapped
packaging
pk of 1 ea
manufacturer/tradename
Ascentis®
parameter
60 °C temp. range, 600 bar max. pressure (9000 psi)
technique(s)
HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable
L × I.D.
10 cm × 3 mm
surface area
135 m2/g
impurities
<5 ppm metals
matrix
Fused-Core particle platform, superficially porous particle
matrix active group
phenylhexyl phase
particle size
2.7 μm
pore size
90 Å
pH range
2-9
application(s)
food and beverages
separation technique
reversed phase
General description
The Phenyl-Hexyl phase has unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity. The Phenyl-Hexyl phase also tend to exhibit good shape selectivity, which may originate from solute multipoint interaction with the planar ring system. More retention and selectivity will often be observed for solutes with aromatic electron-withdrawing groups (fluorine, nitro, etc.) or with a delocalized heterocyclic ring system such as the benzodiazepine compounds.
Application
- A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE-HPLC for determination of ochratoxin A and citrinin in lager beers.: This study presents a rapid, automated method for the detection of mycotoxins in beer using high-performance liquid chromatography. The use of the Ascentis® Express Phenyl-Hexyl column enables efficient and effective sample processing, demonstrating its applicability in food safety and quality control (Lhotska et al., 2016).
- Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids.: This paper explores the benefits of using core-shell particle technology, including Ascentis® Express Phenyl-Hexyl columns, in the analysis of phenolic acids. The study highlights the columns′ enhanced performance and efficiency, making them valuable for chemical analysis in environmental and food samples (Chocholous et al., 2013).
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenu apparenté
Ascentis Express family of HPLC Columns
C M Chavez-Eng et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1011, 204-214 (2016-01-17)
An ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of (4S,5R)-5-[3,5-bis (trifluoromethyl)phenyl]-3-{[4'-fluoro-5'-isopropyl-2'-methoxy-4-(trifluoromethyl)biphenyl-2-yl] methyl}-4-methyl-1,3-oxazolidin-2-one (anacetrapib, I) and [(13)C5(15)N]-anacetrapib, II in human plasma has been developed to support a clinical study to determine the absolute bioavailability of I.
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
Petr Chocholouš et al.
Talanta, 103, 221-227 (2012-12-04)
Currently, for Sequential Injection Chromatography (SIC), only reversed phase C18 columns have been used for chromatographic separations. This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of