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70236 BL21(DE3)pLysS Singles™ Competent Cells - Novagen

70236
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      Description
      OverviewBL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.

      DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

      pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
      Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysS (CamR)


      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Catalogue Number70236
      Brand Family Novagen®
      References
      Product Information
      Components
      Guaranteed efficiencyGuaranteed efficiency: > 2 x 10⁷ cfu/µg
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
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      Documentation

      BL21(DE3)pLysS Singles™ Competent Cells - Novagen Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      BL21(DE3)pLysS Singles™ Competent Cells - Novagen Certificados de análisis

      CargoNúmero de lote
      70236

      Folleto

      Cargo
      The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

      Citas

      Título
    • Laurent R Chiarelli, et al. (2005) Functional analysis of pyrimidine 5'-nucleotidase mutants causing nonspherocytic hemolytic anemia. Blood 105, 3340-3345.
    • Sung Hoon Kim, et al. (2005) A proteomic microarray approach for exploring ligand-initiated nuclear hormone receptor pharmacology, receptor-selectivity,and heterodimer functionality. Molecular and Cellular Proteomics 4, 267-277.
    • Subhradip Mukhopadhyay, et al. (2005) Identification of a region of fast skeletal troponin T required for stabilization of the coiled-coil formation with troponin I. Journal of Biological Chemistry 280, 538-547.
    • Martin Sprick, et al. (2004) cFLIPL inhibits tumor necrosis factor-related apoptosis-inducing ligand-mediated NF-κB activation at the death-inducing signaling complex in human keratinocytes. Journal of Biological Chemistry 279, 52824-52834.
    • Protocolos de usuario

      Cargo
      TB055 pET System Manual

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      Categorías

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression