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MCYT1-190K MILLIPLEX® Mouse Cytokine/Chemokine/Growth Factor Expanded Panel 1

MILLIPLEX® Qualified assays undergo rigourous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 68 analytes in mouse serum, plasma, and cell culture supernatants.

The following analytes in this kit are not compatible with the MAGPIX® System: sFasL, IL-16, sFas, IL-20, sCD137, sICAM-1, CXCL16, sRAGE, CCL25/TECK, BAFF, GDF-15, FLT3L, CHI3L1, MCP-2, MCP-3, EPO, Fracktalkine, IL-31, and Betacellulin.

MCYT1-190K

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Description
Catalogue NumberMCYT1-190K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Mouse Cytokine/Chemokine/Growth Factor Expanded Panel 1
OverviewMILLIPLEX® kits offer the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the verification process include cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability).

Each MILLIPLEX® kit includes:
• Quality controls (QCs) to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Detection antibody cocktails designed to yield consistent analyte profiles within panel

In addition, each panel and kit meet stringent manufacturing criteria to ensure batch to-batch reproducibility. MILLIPLEX® Mouse Cytokine/Chemokine/Growth Factor Expanded Panel 1 is part of the most versatile system available for exosome research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically verify, and build the most comprehensive library available for protein detection and quantitation.
Background InformationMouse models are fundamental in the field of biomedical research, serving as essential tools in preclinical and translational studies across various fields such as immunology, cancer, and infectious diseases. Cytokines, chemokines, and growth factors are key mediators of immune system functions capable of signaling through autocrine, paracrine, and endocrine mechanisms.  Their pleiotropic immunomodulatory properties allow these biomolecules to react to diverse stimuli and regulate the immune response, either promoting or inhibiting inflammation.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: <10%
  • Inter-assay: <15% (Exodus <20%)
Panel TypeMAGNETIC Circulating Cancer
Quality LevelMQ200
Applications
ApplicationMILLIPLEX® Qualified assays undergo rigourous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 68 analytes in mouse serum, plasma, and cell culture supernatants.

The following analytes in this kit are not compatible with the MAGPIX® System: sFasL, IL-16, sFas, IL-20, sCD137, sICAM-1, CXCL16, sRAGE, CCL25/TECK, BAFF, GDF-15, FLT3L, CHI3L1, MCP-2, MCP-3, EPO, Fracktalkine, IL-31, and Betacellulin.
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay.
• This assay requires 25 µL of 1:2 diluted plasma or serum 25 μL cell culture supernatant per well.
• This kit may be used for the analysis of any or all of the Mouse Cytokine/Chemokine/Growth Factor Expanded Panel 1 analytes in mouse tissue/cell lysate and culture supernatant samples and serum or plasma samples.
Biological Information
Species Reactivity
  • Mouse
Analytes Available
  • EGF
  • Eotaxin/CCL11
  • FGF-2
  • G-CSF
  • IFN-α
  • IFNβ
  • IFNγ
  • IL-10
  • IL-12 (p40)
  • IL-12(p70)
  • IL-13
  • IL-15
  • IL-17
  • IL-17F
  • IL-18
  • IL-1α
  • IL-1β
  • IL-2
  • IL-22
  • IL-28B/IFNλ3
  • IL-3
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-9
  • IP-10/CXCL10
  • KC/CXCL1
  • LIF
  • LIX
  • MCP-1/CCL2
  • M-CSF
  • MIG
  • MIP-1α
  • MIP-1β
  • RANTES
  • TNFα
  • VEGF-A
  • IL-33
  • MIP-2
  • BAFF/Blys
  • Betacellulin
  • CXCL6/GCP-2
  • Erythropoietin (EPO)
  • Exodus-2
  • FLT3
  • Fractalkine/CX3CL1
  • GDF-15
  • Granzyme B
  • IL-12
  • IL-16
  • IL-17A
  • IL-20
  • IL-31
  • MCP-3 (CCL7)
  • MCP-5/CCL12
  • MDC (CCL22)
  • MIP-3α/CCL20
  • sCD137
  • sFas
  • sFasL
  • sICAM-1
  • sRAGE
  • TARC/CCL17
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities for individual analytes.
Standard Curve Range
  • Refer to kit protocol for standard curve ranges for individual analytes.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MCYT1-190K 04065272030342

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