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539253 Protein A, Peroxidase Conjugate

539253
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US1539253-1MG
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      Description
      OverviewNative protein A conjugated to horseradish peroxidase.
      Catalogue Number539253
      Brand Family Calbiochem®
      References
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 0.5 mM EDTA, 10% mannose, 2% sucrose, 1% BSA, pH 7.6.
      Preservative0.02% thimerosal
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesDirect Immunoassay (1:10,000)
      Application Commentso-Phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea hydrogen peroxide at 25°C was used as a substrate for testing conjugate enzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      PuritySingle band 50,000 on SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Mercury prohibited To comply with ban of sale of mercury-added products required by The Interstate Mercury Education and Reduction Clearinghouse (IMERC), this product is prohibited to be sold in the following US states: Rhode Island and Connecticut.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protein A, Peroxidase Conjugate Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Protein A, Peroxidase Conjugate Certificados de análisis

      CargoNúmero de lote
      539253
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-August-2007 RFH
      ApplicationDirect Immunoassay (1:10,000)
      DescriptionNative protein A from Staphylococcus aureus conjugated to horseradish peroxidase.
      BackgroundProtein A is the universal secondary antibody because it binds to the Fc portions of most immunoglobulin classes. It is a versatile and economic means to isolate, detect and purify antibodies produced by many animals. It is especially useful in immunohistochemical studies because, unlike secondary antibodies, it does not bind to cell Fc receptors and therefore will not give high background reactions.
      FormLiquid
      FormulationIn 10 mM PBS, 0.5 mM EDTA, 10% mannose, 2% sucrose, 1% BSA, pH 7.6.
      Concentration Label Please refer to vial label for lot-specific concentration
      PuritySingle band 50,000 on SDS-PAGE
      Preservative0.02% thimerosal
      Commentso-Phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea hydrogen peroxide at 25°C was used as a substrate for testing conjugate enzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling