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560000 SPUTOLYSIN® Reagent - CAS 578517 - Calbiochem

560000
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CAS #
3483-12-3

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      Description
      OverviewConcentrate of Dithiothreitol (DTT; Cat. No. 233155) in phosphate buffer, pH 6.5-7.5. Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum. Each vial is sufficient to make 100 ml of working solution.
      Note: 1 set = 20 x 10 ml.
      Catalogue Number560000
      Brand Family Calbiochem®
      References
      ReferencesHirsch, S.R., et al. 1969. J. Lab. Clin. Med. 74, 346.
      Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis. 94, 454.
      Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
      Cleland, W.W. 1964. Biochemistry 3, 480.
      May, J.R. 1953 (September). Lancet p. 535.
      Product Information
      CAS number3483-12-3
      FormLiquid
      Formulation20 vials each containing 10 ml of concentrated DTT OR 1 vial containing 10 ml of concentrated DTT.
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSEK1610000
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +15°C to +30°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsOnce a vial is opened the unused concentrate should be purged with an inert gas (e.g. N₂) and refrigerated (4°C). Diluted solutions, stored aseptically under an inert gas, are stable for up to 48 h at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SPUTOLYSIN® Reagent - CAS 578517 - Calbiochem Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      SPUTOLYSIN® Reagent - CAS 578517 - Calbiochem Certificados de análisis

      CargoNúmero de lote
      560000

      Referencias bibliográficas

      Visión general referencias
      Hirsch, S.R., et al. 1969. J. Lab. Clin. Med. 74, 346.
      Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis. 94, 454.
      Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
      Cleland, W.W. 1964. Biochemistry 3, 480.
      May, J.R. 1953 (September). Lancet p. 535.

      Citas

      Título
    • I.S. Woolhouse, D.L. Bayley and R.A. Stockley. (2002) Effect of Sputum Processing with Dithiothreitol on the Detectino of Inflammatory Mediators in Chronic Bronchitis and Bronchiectasis. Thorax 57, 667-671.
    • Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision28-April-2008 RFH
      DescriptionA concentrate of dithiothreitol (DTT) in phosphate buffer. Useful for the isolation of pathogenic or saprophytic bacteria, fungi, yeast, and epithelial cells from sputum and other mucoid fluids.
      FormLiquid
      Formulation20 vials each containing 10 ml of concentrated DTT OR 1 vial containing 10 ml of concentrated DTT.
      Recommended reaction conditionsA. To prepare sputum samples: 1. In a screw-cap centrifuge tube, overlay the sputum sample with an equal volume of the diluted SPUTOLYSIN® Reagent. 2. Suspend the sputum by mixing on a vortex mixer for 30 s. 3. Allow the suspended mixture to stand at room temperature for 15 min. 4. Centrifuge the mixture for 5 min at 1500 rpm. Discard the supernatant. B. To prepare cultures for acid-fast bacilli: 1. Suspend the pellet from Step A(4) in 5 to 10 ml of 1% sodium hydroxide by mixing on a vortex mixer for 1 min. 2. Centrifuge the mixture for 15 min at 3000 rpm. Discard the supernatant. 3. Rinse the pellet twice in 10 ml of sterile normal saline to remove the residual sodium hydroxide. 4. Resuspend the pellet in 0.5 ml of the diluted SPUTOLYSIN® Reagent.
      CAS number3483-12-3
      RTECSEK1610000
      SolubilityDilute the contents of one vial to 100 ml with sterile distilled water. Swirl gently to dissolve any crystals. If the diluted material appears cloudy, the solution may be filtered to remove any insoluble particles. The diluted solution is ~6.5 mM dithiothreitol in 100 mM phosphate buffer, pH 7.0.
      Storage +15°C to +30°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsOnce a vial is opened the unused concentrate should be purged with an inert gas (e.g. N₂) and refrigerated (4°C). Diluted solutions, stored aseptically under an inert gas, are stable for up to 48 h at 4°C.
      Toxicity Harmful
      ReferencesHirsch, S.R., et al. 1969. J. Lab. Clin. Med. 74, 346.
      Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis. 94, 454.
      Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
      Cleland, W.W. 1964. Biochemistry 3, 480.
      May, J.R. 1953 (September). Lancet p. 535.
      Citation
    • I.S. Woolhouse, D.L. Bayley and R.A. Stockley. (2002) Effect of Sputum Processing with Dithiothreitol on the Detectino of Inflammatory Mediators in Chronic Bronchitis and Bronchiectasis. Thorax 57, 667-671.