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69910 pET-30b(+) DNA - Novagen

69910
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      Description
      OverviewThe pET-30a-c(+) vectors carry an N-terminal His•Tag®/thrombin/S•Tag™/enterokinase configuration plus an optional C-terminal His•Tag sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB095). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.

      The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number69910
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
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      Documentation

      pET-30b(+) DNA - Novagen Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      pET-30b(+) DNA - Novagen Certificados de análisis

      CargoNúmero de lote
      69910

      Citas

      Título
    • Gena D. Tribble, et al. (2006) A Porphyromonas gingivalis haloacid dehalogenase family phosphatase interacts with human phosphoproteins and is important for invasion. Procedings of the National Academy of Science 103, 11027-11032.
    • Sanghamitra Dey, Gregory A. Grant and James C. Sacchettini. (2005) Crystal structure of mycobacterium tuberculosis D-3-phosphoglycerate dehydrogenase: extreme asymmetry in a tetramer of identical subunits. Journal of Biological Chemistry 280, 14892-14899.
    • Akari Fukuda, et al. (2005) Differential distribution of proteins expressed in companion cells in the sieve element-companion cell complex of rice plants. Plant and Cell Physiology 46, 1779-1786.
    • Patricia L. Herman, et al. (2005) A three component dicamba O-demethylase from Pseudomonas maltophilia, strain DI-6: Gene isolation, characterization, and heterologous expression. Journal of Biological Chemistry 280, 24759-24767.
    • Satoru Moritoh, et al. (2005) RNAi-mediated silencing of OsGEN-L (OsGEN-like), a new member of the RAD2/XPG nuclease family, causes male sterility by defect of microspore development in rice. Plant and Cell Physiology 46, 699-715.
    • Saloumeh Kadkhodayan, et al. (2000) Cloning, expression, and one-step purification of the minimal essential domain of the light chain of botulinum neurotoxin type A. Protein Expression and Purification 19, 125-130.
    • H. Uchida, J. Zhang and D.S.D. Nimer. (1997) AML1A and AML1B can transactivate the human IL-3 promoter. Journal of Immunology 158, 2251-2258.
    • B. Huang, et al. (1996) NMR structure and mutagenesis of the Fas (APO-1/CD95) death domain. 384, 638-641.
    • Protocolos de usuario

      Cargo
      TB055 pET System Manual

      Mapa vectorial

      Cargo
      TB095 pET-30a-c(+) Vector Map

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      Número de referencia Descripción  
      69909 pET-30a(+) DNA - Novagen Precios y disponibilidad
      69911 pET-30c(+) DNA - Novagen Precios y disponibilidad

      Categorías

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors