Quality Level
form
suspension (20% aqueous ethanol)
technique(s)
protein array: suitable
matrix
cross-linked agarose
particle size
24-44 μm
pore size
1-100 kDa MW range (dextrans), 10-600 kDa MW range (proteins)
operating pH range
1-14(short term), 3-12(long term)
storage temp.
2-8°C
General description
Combines the high selectivity of a Sephadex matrix with the chemical and physical stability of crosslinked agarose.
Application
Optimized for high resolution preparative separations by size exclusion chromatography. Recommended fractionation ranges are listed for globular proteins and dextrans.
Superdex® is used for protein chromatography, gel filtration chromatography, gel filtration media, resins and separation media. Superdex® has been used for the purification and characterization of a novel laccase from the edible mushroom Hericium coralloides. Superdex® has also been used for the purification and mechanism exploration of a potential human hepatocellular carcinoma inhibitor from Bauhinia purpurea L. seeds.
Legal Information
Superdex is a registered trademark of Cytiva
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Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Storage Class
3 - Flammable liquids
wgk
WGK 3
flash_point_f
104.0 °F
flash_point_c
40 °C
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J Oh et al.
Human molecular genetics, 9(3), 375-385 (2000-02-03)
Hermansky-Pudlak syndrome (HPS) is a rare autosomal recessive disorder in which oculocutaneous albinism, bleeding tendency and a ceroid-lipofuscin lysosomal storage disease result from defects of multiple cytoplasmic organelles: melanosomes, platelet dense granules and lysosomes. The HPS polypeptide, a 700 amino
Thomas Ouellette et al.
Protein expression and purification, 30(2), 156-166 (2003-07-26)
A recombinant form of human rhIL-7 was overexpressed in Escherichia coli HMS174 (DE3) pLysS under the control of a T7 promoter. The resulting insoluble inclusion bodies were separated from cellular debris by cross-flow filtration and solubilized by homogenization with 6
Mario Jiz et al.
Infection and immunity, 76(7), 3164-3169 (2008-04-23)
Despite effective chemotherapy, schistosomiasis remains a major public health problem in the developing world, with at least 200 million active infections resulting in significant morbidity. Rapid reinfection after treatment, accompanied by extensive residual morbidity, mandates alternative control strategies, including vaccine
