This hands-on course provides a fundamental understanding of chromatographic methods and strategies to purify monoclonal antibodies and related molecules. The combination of theoretical lectures, industrial case studies and experiments in the lab enables participants to gain a profound knowledge of biochromatography processes.
- Registration and Welcome
- Basic principles of antibody purification
- Antibody structure
- Protein A affinity chromatography
- Antibody purification by ion-exchange chromatography
- Static and dynamic binding capacities
- Practical, part I
- Capture of antibodies by Protein-A affinity chromatography
- Ion-exchange chromatography for antibody purification
- Determination of dynamic IgG binding capacities
- Analysis and discussion of results
- Different chromatographic techniques for the purification of antibodies: Hydrophobic interaction chromatography, hydroxyapatite & mixed mode chromatography, size exclusion chromatography
- Case studies:
- Regulatory aspects in protein purification and impurities testing (Dr. Bettina Katterle)
- Practical, part II:
- Design of an antibody purification process
- Purification strategies and sequencing of purification steps
- Post-Protein-A – steps in antibody purification
- Optimization of intermediate purification steps
- Optimization of polishing steps
Due to the hands-on nature of this course, enrollment is limited to 8. We recommend early booking to ensure a place.
- Case study:
- Production of monoclonal antibodies ( Xavier Le Saoût)
- Scale up in chromatography
- Presentation and discussion of results
- Course summary and final discussion
For help with registration, hotel accommodation etc., please contact Mrs. Andrea Bentz, Assistant to Prof. Dr. C. Frech (Email: email@example.com).
For more information or to register for this course, please contact firstname.lastname@example.org
Our highly skilled and qualified instructors are experts in chromatography, and combine technical knowledge with field experience and teaching capabilities.
The balance between theory and practice extends to our instructors who do not spend all their time in the theoretical environment of the classroom; they are also working engineers who deal with real applications
on a daily basis.