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Operator certification for chromatography
column packing

Two-day Course

Course Description

Using a combination of theory, workshops and hands-on practical sessions, this course provides a review of key chromatography process steps and focuses on the methodology to successfully combine equipment with chromatographic media to give reproducible column and separation performance.

Catalog number: TRCHROPCER
Course Content
  • Explain the fundamentals of chromatography required to properly pack chromatography columns and assess their performance.
  • Identify the components required in well-designed chromatography columns and their influence on column performance.
  • Pack and unpack different-sized columns under GMP-compliant conditions.
  • Employ several methods for manual and automatic column packing.
  • Use advanced troubleshooting techniques to review column and media performance.
  • Identify the causes of poor or irreproducible column performance.
  • Apply suggested preventive actions when there are column performance issues.
Learning Goals
  • Understanding chromatography column components and their correct operation and maintenance
  • Insufficient product purity and low process yield due to incorrect column packing
  • Lost time due to diagnosing and repacking poorly performing columns
Who should attend?

The course is designed primarily for operators and engineers responsible for chromatographic column operation in the production of a reliable and reproducible product.

What will you receive?
  • A copy of the course materials.
  • A certificate of successful completion. The assessment comes from the results of the workshop and a practical quiz.
Course Schedule and Program
Day 1: 8:30 - 18:30
  • Welcome and course introduction
  • Review of chromatography fundamentals
  • Demonstration - components, column packing and disassembly
  • Column packing
  • Chromatography systems and columns
  • Practical session
Day 2: 8.30 - 17:30
  • Column troubleshooting
  • Practical sessions
  • Certification test
  • Conclusion
Hands-on practical section
The practical sessions use a range of chromatography columns and media commonly found in today’s biopharmaceutical processes and cover how to:
  • Manually pack and unpack lab pilot-scale columns
  • Automatically pack and unpack an industrial column
  • Measure and check column efficiency
Enrollment limited to 9 participants
Due to the hands-on nature of this course, enrollment is limited to 9. We recommend early booking to ensure a place.
Please contact your local representative or email for a quote.