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SCR151 AldeGreen™ ALDH Detection Assay

Live cancer stem cell assay that uses a green-shifted fluorescent dye for ALDH that allows concurrent use of other red fluorescent reporters or probes.

SCR151
1 Kit  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCR151
      DescriptionAldeGreen™ ALDH Detection Assay
      Background InformationThe AldeGreen™ ALDH Detection Assay enables flow cytometry-based detection of intracellular Aldehyde Dehydrogenase (ALDH) enzyme activity. High ALDH activity serves as a universal marker of stem cells, both normal and malignant. The assay employs a fluorescent and non-toxic ALDH substrate (AldeGreen) that diffuses freely into intact and viable cells, but remains trapped inside the cells once converted by ALDH into the corresponding acid. The amount of fluorescence produced is proportional to the ALDH activity in the cells and is measured by flow cytometry, allowing fluorescence-activated cell sorting (FACS). This kit supplies the ALDH inhibitor diethylaminobenzaldehyde (DEAB), which is used in negative control testing necessary for background fluorescence assessment.
      References
      Product Information
      Components
      • 1. AldeGreen™ Reagent: (Part No. CS226325). One (1) vial containing 50 µg of AldeGreen™ reagent powder.
      • 2. DEAB Reagent: (Part No. CS216595). One (1) vial containing 1 mL of DEAB (Diethylaminobenzaldehyde) reagent.
      • 3. Hydrochloric Acid (2N): (Part No. CS216594). One (1) vial containing 1.5 mL of 2 Normal hydrochloric acid.
      • 4. Dimethylsulphoxide: (Part No. CS216593). One (1) vial containing 1.5 mL of Dimethylsulphoxide (DMSO).
      • 5. AldeGreen™ Assay Buffer: (Part No. CS226324). Four (4) bottles containing 25 mL of AldeGreen™ Assay Buffer each.
      • 6. Verapamil: (Part No. CS220012). Four (4) vials containing 615 µg Verapamil powder each.
      Detection methodFluorescence
      Applications
      ApplicationLive cancer stem cell assay that uses a green-shifted fluorescent dye for ALDH that allows concurrent use of other red fluorescent reporters or probes.
      Key Applications
      • Flow Cytometry
      • Fluorescence Activated Cell Sorting (FACS)
      Application Notes• AldeGreen-positive cells may be imaged using a fluorescence microscope or quantified using a flow cytometer. A 488 nm argon ion laser may be used for excitation and an optical filter set to 515-545 nm emission for detection.
      • All live intact cells (even with low ALDH expression) become fluorescent upon exposure to activated AldeGreen. The generation of a DEAB-treated control is necessary for each cell sample to establish baseline fluorescence.
      • Handle all reagents using aseptic techniques.
      Biological Information
      Physicochemical Information
      Emission max.
      • 488 nm
      Excitation max.
      • 515-545 nm
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each lot is tested by measuring ALDH activity in SK-BR-3 cells by flow cytometry.
      • AldeGreen™ Reagent is 95% pure (PMR)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions• Store kit at 2-8°C.
      • Reconstituted and activated AldeGreen™ Reagent may be stored in aliquots at -20°C protected from light for up to 1 year. Repeated freezing and thawing is not recommended.
      • AldeGreen™ Assay Buffer supplemented with Verapamil may be stored at 2-8°C in aliquots for up to 3 months.
      Packaging Information
      Material Size1 Kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCR151 04054839736773