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175600 Angiogenin, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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175600
  
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      Overview

      Replacement Information

      Key Spec Table

      Purity
      ≥95% by SDS-PAGE
      Description
      OverviewRecombinant, human angiogenin expressed in E. coli. Exhibits angiogenic and ribonucleolytic activities. Enhances the ability of endothelial cells to digest extracellular matrix components and degrade basement membrane. Has been shown to stimulate capillary and umbilical vein endothelial cells to produce DAG and secrete prostacyclin by phospholipase activation. Biological activity: 1.0 µg protein produces an absorbance change at 260 nm of approximately 2.0 - 3.0 as measured by yeast tRNA ribonucleolytic activity.
      Catalogue Number175600
      Brand Family Calbiochem®
      References
      ReferencesLeland, P.A., et al. 2002. Biochemistry 41, 1343.
      Hatzi, E., et al. 2000. Biochem. Biophys. Res. Commun. 267, 719.
      Hu, G., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 12096.
      Lee, F.S., and Vallee, B.L. 1989. Biochem. Biophys. Res. Commun. 161, 121.
      Kurachi, R., et al. 1985. Biochemistry 24, 5494.
      Product Information
      FormSolid
      FormulationLyophilized from sterile-filtered PBS containing 50 µg BSA/µg Angiogenin.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activity≥1.0 µg protein produces an absorbance change at 260 nm of ~2.0-3.0 as measured based upon its ribonucleolytic activity toward Yeast tRNA.
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxins: ≤100 pg/µg Angiogenin
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to months at -70°C. Avoid freeze/thaw cycles of solutions.
      Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      175600 0

      Documentation

      Angiogenin, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Angiogenin, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      175600

      References

      Reference overview
      Leland, P.A., et al. 2002. Biochemistry 41, 1343.
      Hatzi, E., et al. 2000. Biochem. Biophys. Res. Commun. 267, 719.
      Hu, G., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 12096.
      Lee, F.S., and Vallee, B.L. 1989. Biochem. Biophys. Res. Commun. 161, 121.
      Kurachi, R., et al. 1985. Biochemistry 24, 5494.

      Brochure

      Title
      Angiogenesis and Tumor Metastasis Brochure and Technical Guide
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-November-2009 JSW
      DescriptionRecombinant, human angiogenin expressed in E. coli. A 14 kDa protein with angeogenic and ribonucleolytic activities. Enhances the proteolytic and fibrinolytic activities of endothelial cells. Also, stimulates the endothelial cell invasion of Matrigel basement membrane. Has been shown to stimulate capillary and umbilical vein endothelial cells to produce DAG and secrete prostacyclin by phospholipase activation.
      FormSolid
      FormulationLyophilized from sterile-filtered PBS containing 50 µg BSA/µg Angiogenin.
      Purity≥95% by SDS-PAGE
      ContaminantsEndotoxins: ≤100 pg/µg Angiogenin
      Biological activity≥1.0 µg protein produces an absorbance change at 260 nm of ~2.0-3.0 as measured based upon its ribonucleolytic activity toward Yeast tRNA.
      SolubilityReconstitute to a concentration ≥1 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to months at -70°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesLeland, P.A., et al. 2002. Biochemistry 41, 1343.
      Hatzi, E., et al. 2000. Biochem. Biophys. Res. Commun. 267, 719.
      Hu, G., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 12096.
      Lee, F.S., and Vallee, B.L. 1989. Biochem. Biophys. Res. Commun. 161, 121.
      Kurachi, R., et al. 1985. Biochemistry 24, 5494.