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ABC1463-100UL Ant-Zyxin

Anti-Zyxin Antibody, Cat. No. ABC1463 is a rabbit polyclonal antibody that detects Zyxin and has been tested for use in Immunocytochemistry and Western Blotting.

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ABC1463-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABC1463-100UL
      DescriptionAnt-Zyxin
      Alternate Names
      • Zyxin-2
      Background InformationZyxin (UniProt: Q15942; also known as Zyxin-2) is encoded by the ZYX gene (Gene ID: 7791) in human. Zyxin is an evolutionarily conserved LIM domain protein that acts as an adhesion plaque protein concentrated at sites of cell adhesion and is widely expressed. It has been implicated in cell adhesion, cell motility, and signaling. Zyxin is reported to associate with members of the Enabled (Ena)/vasodilator-stimulated phosphoprotein (VASP) family of cytoskeletal regulators and plays a role in cytoskeletal dynamics and signaling. Zyxin contains three LIM zinc binding domains (aa 384-443; 444-503; and 504-570), which act as a negative regulator of Zyxin-VASP complexes. Also, it contains four proline rich ActA repeats (aa 64-77; 94-108; 115-121; and 127-137) that are known to interact directly with Ena/VASP proteins. Loss of Zyxin results in inability to thicken stress fibers in response to mechanical stimuli. (Ref.: Hoffman LM et al (2003). Mol. Cell Biol. 23(1); 70-79; Moody JD et al. (2009). Biochem. Biophys. Res. Commun. 378(3), 625-628; Yoshigi et al (2005). J. Cell Biol. 171(2), 209-215.).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
      Applications
      ApplicationAnti-Zyxin Antibody, Cat. No. ABC1463 is a rabbit polyclonal antibody that detects Zyxin and has been tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Zyxin in A431 cell lysate.

      Immunofluorescence Analysis: A representative lot detected Zyxin in newborn mouse skin. Hoffman, L.M., et. al. (2003). Mol Cell Biol. 23(1):70-9; Hoffman, L.M., et. al. (2012). Mol Biol Cell. 23(10):1846-59).

      Western Blotting Analysis): A representative lot detected Zyxin in mouse lung, mouse embryo fibroblasts and in human Ewing sarcoma cells.(Chaturvedi, A., et. al. (2014). Mol Biol Cell. 25(18):2695-709; Hoffman, L.M., et. al. (2003). Mol Cell Biol. 23(1):70-9; Hoffman, L.M., et. al. (2012). Mol Biol Cell. 23(10):1846-59; Hoffman, L.M., et. al. (2006). J Cell Biol. 172(5):771-82).

      Immunocytochemistry Analysis: A representative lot detected Zyxin at focal adhesions in mouse fibroblasts and in 4T1 cells with ATG7 shRNA or ATG5 shRNA (Hoffman, L.M., et. al. (2006). J Cell Biol. 172(5):771-82; Sharifi, M.N., et. al. (2016). Cell Rep. 15(8):1660-72).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal half of human Zyxin.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Zyxin in human, mouse, and chicken cells. It targets an epitope within 20 amino acids from the C-terminal half.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      • Chicken
      Species Reactivity NoteHuman, Mouse, Chicken.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ZYX
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~75 kDa observed; 61.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH3T3 cell lysate.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Zyxin in NIH3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC1463-100UL 04054839468896

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