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07-1416 Anti-4E-BP1 Antibody

Anti-4E-BP1 Antibody detects level of 4E-BP1 & has been published & validated for use in WB, IH(P).

Research Category: Signaling Research Sub-Category: PI3K, Akt, & mTOR Signaling Gene Symbol: EIF4EBP1, PHAS-I, MGC4316, 4E-BP1, 4EBP1, BP-1 UniProt Number: Q13541
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07-1416
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, PoWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1416
      Replaces04-321
      DescriptionAnti-4E-BP1 Antibody
      References
      Product Information
      FormatAffinity Purified
      Control
      • A431 cell lysate
      PresentationPurified rabbit polyclonal IgG in buffer containing Tris-citrate/phosphate, pH 7-8, and 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-4E-BP1 Antibody detects level of 4E-BP1 & has been published & validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemisty (paraffin): 4E-BP1 representative staining pattern/morphology in thyroid cancer. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as staining pattern/morphology in thyroid cancer tissue.

      Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.
      Biological Information
      ImmunogenSynthetic peptide corresponding to a portion of human eIF4E Binding Protein encoded in part by exon 2 and 3.
      HostRabbit
      SpecificityEukaryotic translation initiation factor 4E binding protein (eIF4E Binding Protein, eIF4EBP1, 4E-BP1, 4EBP1).
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Pig
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in sponse to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.[provided by RefSeq]
      Gene Symbol
      • EIF4EBP1
      • PHAS-I
      • MGC4316
      • 4E-BP1
      • 4EBP1
      • BP-1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway.
      SUBUNIT: Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.
      PTM: Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: Belongs to the eIF4E-binding protein family.
      Molecular Weight15-20 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on A431 lysates.

      Western Blot Analysis:
      1:500-1:1,000 dilution of this lot detected 4E-BP1 on 10 μg of A431 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-1416 04053252507229

      Documentation