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MABN2411 Anti-8-Nitroguanine Antibody, clone NO2-52

Anti-8-Nitroguanine, clone NO2-52, Cat. No. MABN2411, is a highly specific mouse monoclonal antibody that targets 8-Nitroguanine and has been tested for use in ELISA, Immunocytochemistry, and Western Blotting.

Anti-8-Nitroguanine Antibody, clone NO2-52 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience UniProt Number: N/A
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MABN2411
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MELISA, ICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN2411
      DescriptionAnti-8-Nitroguanine Antibody, clone NO2-52
      Alternate Names
      • 8-NO2-Gua
      Background Information8-Nitroguanosine, a highly reactive redox-active nucleic acid derivative, is formed by the action of peroxynitrite. It is a nitrated base of DNA and RNA formed as a result of chronic inflammation. It has been well established that large amount of nitric oxide molecules and superoxide anion, generated as a result of inflammatory response, can cause nitration of guanosine. Such chemically modified nucleotides increase the incidence of mutation during DNA replication. 8-nitroguanosine is considered as a marker of DNA damage related to mutation and cancer. Nitrated guanine nucleosides and nucleotides in the nucleotide pool are shown to contribute to oxidative stress via production of superoxide mediated by various reductases and may disturb or modulate directly various important proteins and enzymes, such as G-proteins and cGMP-dependent enzymes. Clone NO2-52 is a highly specific monoclonal antibody that recognizes 8-nitroguanine and 8-nitroguanosine. However, it does not cross-react with normal nucleotide bases. (Ref.: Ohshima, H., et al. (2006). Antioxid. Redox Signal 8(5-6); 1033-45; Sawa, T., et al. (2007). Nat. Chem. Biol. 3(11); 727-35).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-8-Nitroguanine, clone NO2-52, Cat. No. MABN2411, is a highly specific mouse monoclonal antibody that targets 8-Nitroguanine and has been tested for use in ELISA, Immunocytochemistry, and Western Blotting.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Western Blotting
      Application NotesELISA Analysis: A representative lot detected 8-Nitroguanine in ELISA applications (Sawa, T., et. al. (2007). Nat Chem Biol. 3(11):727-35; Yoshitake, J., et. al. (2004). J Virol. 78(16):8709-19).

      Immunocytochemistry Analysis: A representative lot detected 8-Nitroguanine in Immunocytochemistry applications (Ihara, H., et. al. (2011). Nitric Oxide. 25(2):169-75; Sawa, T., et. al. (2007). Nat Chem Biol. 3(11):727-35; Yoshitake, J., et. al. (2004). J Virol. 78(16):8709-19).
      Biological Information
      ImmunogenBSA conjugated 8-nitroguanosine.
      CloneNO2-52
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone NO2-52 specifically detects 8-nitroguanine.
      IsotypeIgG1κ
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteMouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodPurified by ion-exchange chromatography
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blottingwith Nitroguanine-conjugated BSA (NG-BSA).

      Western Blotting Analysis: 1 µg/mL of this antibody detected 8-Nitroguanine in Nitroguanine-conjugated BSA (NG-BSA). It does not react with BSA alone.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2411 04054839412394

      Documentation

      Anti-8-Nitroguanine Antibody, clone NO2-52 Certificates of Analysis

      TitleLot Number
      Anti-8-Nitroguanine, clone NO2-52 - 3308161 3308161
      Anti-8-Nitroguanine, clone NO2-52 - 3476009 3476009
      Anti-8-Nitroguanine, clone NO2-52 - 3639206 3639206
      Anti-8-Nitroguanine, clone NO2-52 - 3905547 3905547
      Anti-8-Nitroguanine, clone NO2-52 - 4045890 4045890
      Anti-8-Nitroguanine, clone NO2-52 - 4311818 4311818
      Anti-8-Nitroguanine, clone NO2-52 - 4322329 4322329
      Anti-8-Nitroguanine, clone NO2-52 Monoclonal Antibody Q2964967