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MAB1810 Anti-ADAMTS-1 Antibody, clone 5D4E11B6

Detect ADAMTS-1 using this Anti-ADAMTS-1 Antibody, clone 5D4E11B6 validated for use in ELISA, IP & WB.

Anti-ADAMTS-1 Antibody, clone 5D4E11B6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins, MMPs & TIMPs Gene Symbol: ADAMTS1, KIAA1346, METH1, C3-C5 UniProt Number: Q9UHI8
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MAB1810
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RELISA, IP, WBMSerumMonoclonal Antibody
      Description
      Catalogue NumberMAB1810
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-ADAMTS-1 Antibody, clone 5D4E11B6
      Alternate Names
      • METH-1
      Background InformationADAMTS-1 is a member of a family of extracellular proteases known as ADAMTS (a disintegrin and metalloprotease with thrombospondin motifs). Members of the ADAMTS family share distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TSP) motif. Individual members of this family differ in the number of C-terminal TSP motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TSP motifs. ADAMTS-1 cleaves aggrecan, a cartilage proteoglycan and appears to demonstrate angiogenesis inhibitory activity. The maturation of ADAMTS-1 requires two independent and sequential processing events that may release two forms of the protein. With the cleavage of the prodomain from the 110 kDa zymogen, an 87 kDa active form remains which can be further processed by removal of the catalytic subunit from the TSP repeats, leaving the C-termianl 65 kDa soluble form. The proteolytic deletion of the last two TSP repeats, is probably significant for the in vivo function of this protein.
      References
      Product Information
      FormatSerum
      HS Code3002 15 90
      Control
      • A431 cell lysate
      • HEK293 cell lysate
      • PC-12 cell lysate
      • Embryonic mouse brain lysate
      • MCF-7 cell lysate
      PresentationAscites. Liquid containing 0.05% NaN3.
      Quality LevelMQ100
      Applications
      ApplicationDetect ADAMTS-1 using this Anti-ADAMTS-1 Antibody, clone 5D4E11B6 validated for use in ELISA, IP & WB.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blot: 1:2000 - 1:4000 dilution on A431 cell lysate

      Note: MW bands detected vary by cell line or sample with detection of zymogen and/or active forms.

      Immunoprecipitation: confirm by immunoblot using clone 5D4E11B6; 1:50 using clone 5C6D5 (Catalog # MAB1771) to immunoprecipitate ADAMTS-1 from 500 µg of HEK293 whole cell lysate or PC-12 whole cell lysate
      Biological Information
      ImmunogenMyc-His carboxyl tagged full-length recombinant protein based on the human sequence.
      EpitopeUnknown
      Clone5D4E11B6
      HostMouse
      SpecificityClone 5D4E11B6 recognizes human ADAMTS-1.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteReactivity with other species has not been tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function.
      Gene Symbol
      • ADAMTS1
      • KIAA1346
      • METH1
      • C3-C5
      UniProt Number
      UniProt SummaryFUNCTION: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.
      CATALYTIC ACTIVITY: Cleaves aggrecan at the 1938-Glu-|-Leu-1939 site, within the chondroitin sulfate attachment domain.
      COFACTOR: Binds 1 zinc ion per subunit (By similarity).
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
      DOMAIN: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
      DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
      PTM: The precursor is cleaved by a furin endopeptidase (By similarity).
      SIMILARITY: Contains 1 disintegrin domain and contains 1 peptidase M12B domain and contains 3 TSP type-1 domains.
      Molecular Weight110 / 87 / 65 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C for up to 1 year from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1810 04053252615481

      Documentation

      Anti-ADAMTS-1 Antibody, clone 5D4E11B6 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-ADAMTS-1 Antibody, clone 5D4E11B6 Certificates of Analysis

      TitleLot Number
      Anti-ADAMTS-1, -2682541 2682541
      Anti-ADAMTS-1, clone 5D4E11B5 - 3227946 3227946
      Anti-ADAMTS-1, clone 5D4E11B5 - 3317137 3317137
      Anti-ADAMTS-1, clone 5D4E11B5 - 3422079 3422079
      Anti-ADAMTS-1, clone 5D4E11B5 Monoclonal Antibody 2976878

      References

      Reference overviewPub Med ID
      ADAMTS1/METH1 inhibits endothelial cell proliferation by direct binding and sequestration of VEGF165.
      Luque, Alfonso, et al.
      J. Biol. Chem., 278: 23656-65 (2003)  2003

      		12716911 12716911
      Characterization of METH-1/ADAMTS1 processing reveals two distinct active forms.
      Rodriguez-Manzaneque, J C, et al.
      J. Biol. Chem., 275: 33471-9 (2000)  2000

      		10944521 10944521