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MAB1810-I-25UG Anti-ADAMTS-1 Antibody, clone 5D4E11B6

Anti-ADAMTS-1, clone 5D4E11B6, Cat. No. MAB1810-I, is a mouse monoclonal antibody that detects ADAMTS-1 and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, and Western Blotting.

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MAB1810-I-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB1810-I-25UG
      DescriptionAnti-ADAMTS-1 Antibody, clone 5D4E11B6
      Alternate Names
      • A disintegrin and metalloproteinase with thrombospondin motifs 1
      • ADAM-TS 1
      • ADAM-TS1
      • METH-1
      Background InformationA disintegrin and metalloproteinase with thrombospondin motifs 1 (UniProt: Q9UHI8; also known as ADAM-TS 1, ADAM-TS1, ADAMTS-1, METH-1) is encoded by the ADAMTS1 (also known as KIAA1346, METH1) gene (Gene ID: 9510) in human. ADAM-TS1 is a zinc-binding protein that is synthesized with a signal peptide (aa 1-49) and a propeptide (aa 50-252) that are subsequently cleaved by furin endopeptidase to generate the mature form (aa 253-967). It has a conserved cysteine in the cysteine-switch motif that binds the catalytic zinc ion and inhibits the enzyme. The dissociation of cysteine from the zinc ion upon the activation-peptide release activates the enzyme. It also contains spacer domain (aa 725-849) and the TSO-1 domain that are important for a tight interaction with the extracellular matrix. ADAM-TS1 cleaves aggrecan, a cartilage proteoglycan at Glu1938-Leu1939 site within the chondroitin sulfate attachment domain and is involved in its turnover. ADAMTS1 is implicated in vascularization and development and displays anti- and pro-tumorigenic activities. ADAMTS1 knockout mice exhibit reduced body weight, kidney malformation, and impaired female fertility. (Ref.: Rodriguez-Baena, FJ., et al. (2018). Sci. Rep. 8: Article 13103).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-ADAMTS-1, clone 5D4E11B6, Cat. No. MAB1810-I, is a mouse monoclonal antibody that detects ADAMTS-1 and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • ELISA
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A 1:125 dilution from a representative lot detected ADAMTS-1 in lysates from A431 cells and E16 mouse embryonic brain tissue.

      Western Blotting Analysis: A representative lot detected ADAMTS-1 in Western Blotting applications (Meng, T.G., et. al. (2017). Cell Death Dis. 8(6):e2846; Luque, A., et. al. (2003). J Biol. Chem. 278 (26); 23656-65).

      Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected ADAMTS-1 in HeLa cells.

      ELISA Analysis: A representative lot detected ADAMTS-1 in ELISA applications (Luque, A., et. al. (2003). J Biol Chem. 278(26); 23656-65).

      Immunofluorescence Analysis: A representative lot detected ADAMTS-1 in Immunofluorescence applications (Silva, S.V., et. al. (2016). PLoS One. 11(10):e0165061; Luque, A., et. al. (2003). J Biol Chem. 278(26); 23656-65).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenMyc-His-tagged full length recombinant human ADAMTS-1.
      EpitopeUnknown
      Clone5D4E11B6
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 5D4E11B6 is a mouse monoclonal antibody that detects A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS-1).
      IsotypeIgGκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ADAMTS1
      • KIAA1346
      • METH1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~58 kDa observed; 105.36 kDa calculated for the complete molecule. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: A 1:125 dilution of this antibody detected ADAMTS-1 in HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1810-I-25UG 04065265944892