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NE1041 Anti-AGT Mouse mAb (1A10)

Anti-AGT Mouse mAb (1A10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Angiotensinogen

NE1041
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~50 kDa AGT protein in HepG2 cells.
      Catalogue NumberNE1041
      Brand Family Calbiochem®
      SynonymsAnti-Angiotensinogen
      Application Data
      Detection of human AGT by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-AGT Mouse mAb (1A10) (Cat. No. NE1041) (1-5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesJain S., et al. 2008. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295, R1849.
      Jain, S., et al. 2007. Am. J. Physiol. Cell Physiol. 293, C401.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHepG2 cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 376-485 human AGT, expressed as a GST fusion protein
      Clone1A10
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NE1041 0

      Documentation

      References

      Reference overview
      Jain S., et al. 2008. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295, R1849.
      Jain, S., et al. 2007. Am. J. Physiol. Cell Physiol. 293, C401.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-November-2010 RFH
      SynonymsAnti-Angiotensinogen
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human AGT by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-AGT Mouse mAb (1A10) (Cat. No. NE1041) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~50 kDa AGT protein.
      BackgroundThe protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 376-485 human AGT, expressed as a GST fusion protein
      Clone1A10
      IsotypeIgG₁
      Specieshuman
      Positive controlHepG2 cells
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesJain S., et al. 2008. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295, R1849.
      Jain, S., et al. 2007. Am. J. Physiol. Cell Physiol. 293, C401.