Millipore Sigma Vibrant Logo

ABC40 Anti-AIP1/Alix Antibody

View Products on
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited Availability
Limited Availability
In Stock 
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers


      Contact Customer Service


      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, HWB, IFRbAffinity PurifiedPolyclonal Antibody
      Catalogue NumberABC40
      DescriptionAnti-AIP1/Alix Antibody
      Alternate Names
      • Programmed cell death 6-interacting protein
      • ALG-2-interacting protein 1
      • ALG-2-interacting protein X
      • E2F1-inducible protein
      • Eig2
      Background InformationProgrammed cell death 6-interacting protein (UniProt: Q9WU78; also known as ALG-2-interacting protein 1, ALG-2-interacting protein X, E2F1-inducible protein, Eig2) is encoded by the Pdcd6ip (also known as Aip1, Alix) gene (Gene ID: 18571) in murine species. AIP1/ALIX is a class E VPS cytoplasmic, ubiquitously expressed protein that is involved in concentration and sorting of cargo proteins of the multi-vesicular body (MVB) for incorporation into intra-lumenal vesicles that are generated by invagination and scission from the limiting membrane of the endosome. MVB-mediated sorting is driven by five distinct ESCRT complexes (ESCRT-0, -I, -II and -III, and the Vps4 complex) and associated proteins. AIP1/ALIX and charged MVB protein 4 (CHMP4) are binding partners involved in a variety of ESCRT-III-mediated membrane-remodeling in mammalian cells. In the absence of binding partners, an intramolecular interaction between the Patch 2 region in the BRO1 domain (aa 3-392) and the TSG101-docking site (aa 717-720) in the Pro-rich domain (aa 717-860) locks ALIX in a conformation inaccessible for interaction with CHMP4 and retroviral Gag proteins. AIP1/ALIX interacts with ALG-2 (apoptosis-linked gene-2) to regulate cell death. This interaction is strictly dependent on calcium. It is also known to regulate exosome biogenesis in concert with SDC1/4 and SDCBP.
      Product Information
      FormatAffinity Purified
      • HeLa cell lysate
      PresentationPurified rabbit polyclonal antibody in buffer containing 20 mM Tris-HCl, pH 7.2, 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-AIP1/ALIX , Cat. No. ABC40, is a rabbit polyclonal antibody that detects Programmed cell death 6-interacting protein (AIP1/ALIX) and is tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesWestern Blotting Analysis: A representative lot detected AIP1/ALIX in Western Blotting applications (Polanco, J.C., et. al (2016). J Biol Chem. 291(24):12445-66; Cao, Z., et. al. (2019). Neurosci. 696:114-120; Gauthier, S.A., et. al. (2017). Act Neropathol Commun. 5(1):65).

      Immunofluorescence Analysis: A representative lot detected AIP1/ALIX in Immunofluorescence applications (Harischandra, H. et. al. (2018). PLoS Negl Trop Dis. 12(4):e0006438; Polanco, J.C., et. al (2016). J Biol Chem. 291(24):12445-66).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 479 amino acids from the C-terminal half of mouse AIP1/Alix.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis rabbit polyclonal antibody specifically detects Programmed cell death 6-interacting protein (AIP/ALIX). It targets an epitope within the C-terminal region.
      Species Reactivity
      • Rat
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Pdcd6ip
      • Aip1
      • Alix
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~85 kDa observed; 96.02 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected AIP1/ALIX in lysate from Jurkat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalogue Number GTIN
      ABC40 04053252414534


      Required Licenses


      Anti-AIP1/Alix Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-AIP1/Alix Antibody Certificates of Analysis

      TitleLot Number
      Anti-AIP1/Alix 3069700
      Anti-AIP1/Alix - 2388320 2388320
      Anti-AIP1/Alix - 2461579 2461579
      Anti-AIP1/Alix - 1984695 1984695
      Anti-AIP1/Alix - 2080289 2080289
      Anti-AIP1/Alix - 2109560 2109560
      Anti-AIP1/Alix - 2151865 2151865
      Anti-AIP1/Alix - 2179360 2179360
      Anti-AIP1/Alix - 2210376 2210376
      Anti-AIP1/Alix - 2280480 2280480


      Reference overviewPub Med ID
      α-Synuclein in salivary extracellular vesicles as a potential biomarker of Parkinson's disease
      Zhentang Cao 1 , Yufeng Wu 2 , Genliang Liu 1 , Ying Jiang 1 , Xuemei Wang 1 , Zhan Wang 1 , Tao Feng 3
      Neurosci Lett  163  114-120  2019

      Show Abstract
      30579996 30579996
      Profiling extracellular vesicle release by the filarial nematode Brugia malayi reveals sex-specific differences in cargo and a sensitivity to ivermectin
      Hiruni Harischandra 1 , Wang Yuan 1 , Hannah J Loghry 1 , Mostafa Zamanian 2 , Michael J Kimber 1
      PLoS Negl Trop Dis  12(4)  e0006438  2018

      Show Abstract
      29659599 29659599
      Enhanced exosome secretion in Down syndrome brain - a protective mechanism to alleviate neuronal endosomal abnormalities
      Sébastien A Gauthier 1 , Rocío Pérez-González 1 , Ajay Sharma 1 , Fang-Ke Huang 2 , Melissa J Alldred 1 3 , Monika Pawlik 1 , Gurjinder Kaur 1 , Stephen D Ginsberg 1 3 4 5 , Thomas A Neubert 2 5 , Efrat Levy 6 7 8 9 10
      Acta Neuropathol Commun  5(1)  65  2017

      Show Abstract
      28851452 28851452
      Extracellular Vesicles Isolated from the Brains of rTg4510 Mice Seed Tau Protein Aggregation in a Threshold-dependent Manner
      Juan Carlos Polanco 1, Benjamin James Scicluna 2, Andrew Francis Hill 2, Jürgen Götz 3
      J Biol Chem  291(24)  12445-66  2016

      Show Abstract
      27030011 27030011
      Plasma Exosomal miRNAs in Persons with and without Alzheimer Disease: Altered Expression and Prospects for Biomarkers.
      Lugli, G; Cohen, AM; Bennett, DA; Shah, RC; Fields, CJ; Hernandez, AG; Smalheiser, NR
      PloS one  10  e0139233  2015

      Show Abstract
      26426747 26426747