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AP1052 Anti-APAF-1 Rabbit pAb

Anti-APAF-1 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
AP1052
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~130 kDa APAF-1 protein in HeLa cells.
    • Antibody Target Gene Symbol: APAF1
    • Target Synonym: 6230400I06RIK, APAF1L, CED4, DKFZp781B1145, fog, KIAA0413, mKIAA0413
    • Entrez Gene Name: apoptotic peptidase activating factor 1
    • Hu Entrez ID: 317 (Related Antibodies: AP1038)
    • Mu Entrez ID: 11783
    • Rat Entrez ID: 78963
      		•
      Catalogue NumberAP1052
      Brand Family Calbiochem®
      Application Data
      Detection of human Apaf-1 by immunoblotting. Sample: Whole cell lysate (30 µg) from Hela cells. Primary antibody: Anti-Apaf-1 Rabbit pAb (Cat. No. AP1052) (1 µg/ml). Detection: chemiluminescence
      References
      ReferencesPan, G., et al. 1998. J. Biol Chem. 273, 5841.
      Li, P., et al. 1997. Cell 91, 479.
      Zou, H., et al 1997. Cell 90, 405.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1 µg/ml)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenfull-length, recombinant, human APAF-1 fused to a His•Tag® sequence and expressed in E. coli
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1052 0

      Documentation

      References

      Reference overview
      Pan, G., et al. 1998. J. Biol Chem. 273, 5841.
      Li, P., et al. 1997. Cell 91, 479.
      Zou, H., et al 1997. Cell 90, 405.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-August-2007 RFH
      ApplicationImmunoblotting (1 µg/ml)
      Application Data
      Detection of human Apaf-1 by immunoblotting. Sample: Whole cell lysate (30 µg) from Hela cells. Primary antibody: Anti-Apaf-1 Rabbit pAb (Cat. No. AP1052) (1 µg/ml). Detection: chemiluminescence
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~130 kDa Apaf-1 protein.
      BackgroundThe Apaf-1 (apoptotic peptidase activating factor) gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds to and cleaves caspase-9 proenzyme, releasing mature, activated caspase-9. Activated caspase-9 stimulates the downstream caspase cascade that commits the cell to apoptosis. Alternative splicing of Apaf-1 results in several transcriptional variants encoding different isoforms. Apaf-1 is the ortholog of Ced4 in worms. Alterations in apoptosome function have been documented in a number of tumor types. Modulation of apoptosome function in cancer has been observed in metastatic melanomas in which Apaf-1 protein was lost due to methylation-induced transcriptional silencing.
      HostRabbit
      Immunogen speciesHuman
      Immunogenfull-length, recombinant, human APAF-1 fused to a His•Tag® sequence and expressed in E. coli
      IsotypeIgG
      Specieshuman
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesPan, G., et al. 1998. J. Biol Chem. 273, 5841.
      Li, P., et al. 1997. Cell 91, 479.
      Zou, H., et al 1997. Cell 90, 405.