Millipore Sigma Vibrant Logo

MAB2605 Anti-ATG5 Antibody, clone 177.19

This Anti-ATG5 Antibody, clone 177.19 is validated for use in WB, IP for the detection of ATG5.

Anti-ATG5 Antibody, clone 177.19 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: APG5 , APG5-LIKE , APG5L, ASP, hAPG5 UniProt Number: Q9H1Y0
View Products on Sigmaaldrich.com
MAB2605
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Special Offers

      100% Performance Guaranteed

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB2605
      ReplacesMABC137
      DescriptionAnti-ATG5 Antibody, clone 177.19
      Alternate Names
      • APG5 autophagy 5-like
      • APG5 autophagy 5-like (S. cerevisiae)
      • APG5 (autophagy 5, S. cerevisiae)-like
      • ATG5 autophagy related 5 homolog (S. cerevisiae)
      • ATG5 autophagy related 5-like
      • Apoptosis-specific protein
      Background InformationATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity
      References
      Product Information
      FormatPurified
      Control
      • Western Blot and Immunoprecipitation: HeLa cell lysate.
      PresentationPurified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-ATG5 Antibody, clone 177.19 is validated for use in WB, IP for the detection of ATG5.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation: HeLa cell lysate
      Biological Information
      ImmunogenLinear Peptide, aa: 8-318
      Clone177.19
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody reacts with the 50 kDa ATG5 protein.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteReacts with human. Other species have not been tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • APG5
      • APG5-LIKE
      • APG5L
      • ASP
      • hAPG5
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed By similarity.
      May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
      SUBCELLULAR LOCATION: Cytoplasm. Note= Colocalizes with nonmuscle actin.
      TISSUE SPECIFICTY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
      INDUCTION: By apoptotic stimuli.
      PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
      SEQUENCE SIMILARITIES: Belongs to the ATG5 family
      Molecular Weight50 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis: 0.5 ug/mL of this antibody detected ATG5 on HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB2605 04053252398391

      Documentation

      Anti-ATG5 Antibody, clone 177.19 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-ATG5 Antibody, clone 177.19 Certificates of Analysis

      TitleLot Number
      Anti-ATG5, clone 177.19 - 2370811 2370811
      Anti-ATG5, clone 177.19 - 2437375 2437375
      Anti-ATG5, clone 177.19 - 3193903 3193903
      Anti-ATG5, clone 177.19 - NG1594512 NG1594512
      Anti-ATG5, clone 177.19 - NG1820223 NG1820223
      Anti-ATG5, clone 177.19 - NG1895551 NG1895551
      Anti-ATG5, clone 177.19 Monoclonal Antibody 2985064

      Brochure

      Title
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression

      Newsletters / Publications

      Title
      Autophagy Research Focus