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ST1559 Anti-ATP7B Mouse mAb (3E10)

ST1559
  
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      Overview

      Replacement Information

      Key Spec Table

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      Description
      Overview

      This product has been discontinued.





      Recognizes the ~200 kDa ATP7B protein in human colon tissue.
      Catalogue NumberST1559
      Brand Family Calbiochem®
      SynonymsAnti-ATPase, Copper Transporting, β Polypeptide
      Application Data
      Detection of human ATP7B by immunoblotting. Sample: Whole tissue extract fom human colon. Primary antibody: Anti-ATP7B Mouse mAb (3E10) (Cat. No. ST1559) (1-5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesAnsede, J,H., et al. 2009. Drug Metab. Dispos. 37, 969.
      Product Information
      FormLiquid
      FormulationIn PBS pH 7.20.
      Positive controlHuman colon tissue
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 1372-1466 human ATP7B, expressed as a GST fusion protein
      Clone3E10
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      References

      Reference overview
      Ansede, J,H., et al. 2009. Drug Metab. Dispos. 37, 969.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-November-2010 RFH
      SynonymsAnti-ATPase, Copper Transporting, β Polypeptide
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human ATP7B by immunoblotting. Sample: Whole tissue extract fom human colon. Primary antibody: Anti-ATP7B Mouse mAb (3E10) (Cat. No. ST1559) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~200 kDa ATP7B protein.
      BackgroundThis protein is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD).
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 1372-1466 human ATP7B, expressed as a GST fusion protein
      Clone3E10
      IsotypeIgG₁
      Specieshuman
      Positive controlHuman colon tissue
      FormLiquid
      FormulationIn PBS pH 7.20.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesAnsede, J,H., et al. 2009. Drug Metab. Dispos. 37, 969.