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AB5674P Anti-Acid Sensitive Ion Channel 1 Antibody

Detect Acid Sensitive Ion Channel 1 using this Anti-Acid Sensitive Ion Channel 1 Antibody validated for use in ELISA & WB.

Anti-Acid Sensitive Ion Channel 1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: ACCN2, BNaC2, ASIC1A, hBNaC2, ASIC1, ASIC, BNAC2 UniProt Number: P78348
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AB5674P
50 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RELISA, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5674P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Acid Sensitive Ion Channel 1 Antibody
      Alternate Names
      • ASIC1
      • BNC1
      • MDEG
      • BNaC1a
      • Amiloride-sensitive Cation Channel 2
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Liquid in phosphate buffered saline containing 0.1% BSA.
      Quality LevelMQ100
      Applications
      ApplicationDetect Acid Sensitive Ion Channel 1 using this Anti-Acid Sensitive Ion Channel 1 Antibody validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern blot: 1-10 μg/mL using ECL.

      Immunohistochemistry: not tested. It is recommended that the antibody be tried at 1-20 μg/mL on paraformaldehyde fixed tissue.

      ELISA: 0.5-1 μg/mL using 1 μg/mL control peptide (Catalog number AG394) per well.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenA 20 amino acid peptide sequence near the extracellular N-terminus of rat ASIC1 (Chen et al. 1998; Waldmann et al. 1997; Garcia-Anoveros et al. 1997; Waldmann et al. 1996).

      The immunogen sequence has 100% homology with human sodium channel 2 or BNaC2.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes rat Acid Sensitive Ion Channel 1 (ASIC1). The immunogen shows no significant sequence homology with ASIC-beta or other ASICs.
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown. Alternative splicing of this gene generates 2 transcript products.
      Gene Symbol
      • ACCN2
      • BNaC2
      • ASIC1A
      • hBNaC2
      • ASIC1
      • ASIC
      • BNAC2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P78348 # Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear (By similarity).
      SIZE: 528 amino acids; 59967 Da
      SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable). Interacts with STOM and ACCN1 (By similarity). Interacts with PRKCABP.
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Note=Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4 (By similarity).
      TISSUE SPECIFICITY: Expressed in most or all neurons.
      PTM: Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel.
      SIMILARITY: SwissProt: P78348 ## Belongs to the amiloride-sensitive sodium channel family.
      MISCELLANEOUS: Potentiated by Ca(2+), Mg(2+), Ba(2+) and multivalent cations. Inhibited by anti-inflammatory drugs like salicylic acid (By similarity). Potentiated by FMRFamide-related neuropeptides. PH dependence may be regulated by serine proteases.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB5674P 04053252405402

      Documentation

      Anti-Acid Sensitive Ion Channel 1 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Acid Sensitive Ion Channel 1 Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-ACID SENSITIVE ION CHANNEL 1 AB5674P
      RABBIT ANTI-ACID SENSITIVE ION CHANNEL 1 (ASIC1, BnaC2) - 3852247 3852247
      RABBIT ANTI-ACID SENSITIVE ION CHANNEL 1 (ASIC1, BnaC2) - 4015627 4015627
      RABBIT ANTI-ACID SENSITIVE ION CHANNEL 1 (ASIC1, BnaC2) - 4277499 4277499
      RABBIT ANTI-ACID SENSITIVE ION CHANNEL 1 (ASIC1, BnaC2) -2567100 2567100
      RABBIT ANTI-ACID SENSITIVE ION CHANNEL 1 (ASIC1, BnaC2) -2818276 2818276
      RABBIT ANTI-ACID SENSITIVE ION CHANNEL 1 (ASIC1, BnaC2) AFFINITY PURIFIED POLYCLONAL ANTIBODY 2884803
      RABBIT ANTI-ACID SENSITIVE ION CHANNEL 1 (ASIC1, BnaC2) AFFINITY PURIFIED POLYCLONAL ANTIBODY 2895107
      RABBIT ANTI-ACID SENSITIVE ION CHANNEL 1 (ASIC1, BnaC2) AFFINITY PURIFIED POLYCLONAL ANTIBODY 2876886
      RABBIT ANTI-ACID SENSITIVE ION CHANNEL 1 (ASIC1, BnaC2) AFFINITY PURIFIED POLYCLONAL ANTIBODY 3099985
      Show more

      Brochure

      Title
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

      Data Sheet

      Title
      RABBIT ANTI-ACID SENSITIVE ION CHANNEL 1 (ASIC1, BnaC2) AFFINITY PURIFIED POLYCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies