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CP64 Anti-Actin (Ab-3) Mouse mAb (HHF-35)

Anti-Actin (Ab-3) Mouse mAb (HHF-35) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CP64
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.



      We are offering Anti-Actin Antibody, clone C4 (Cat. No. MAB1501) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.






      Recognizes the ~42 kDa α and γ actin proteins in skeletal, cardiac, and smooth muscle cells.
    • Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1
    • Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN
    • Entrez Gene Name: actin, alpha 1, skeletal muscle
    • Hu Entrez ID: 58, 71 (Related Antibodies: CP01)
    • Mu Entrez ID: 11459 ;-; 11465
    • Rat Entrez ID: 29437 ;-; 287876
      		•
      Catalogue NumberCP64
      Brand Family Calbiochem®
      Application Data
      Detection of actin by staining paraffin sections. Sample: Skeletal muscle. Primary antibody: Anti-Actin (Ab-3) Mouse mAb (HHF-35) (Cat. No. CP64) Detection: colorimetric (DAB).
      References
      ReferencesSchmidt, R.A., et al. 1988. Am. J. Pathol. 131, 19.
      Tsukada, T., et al. 1987. Am. J. Pathol. 127, 389.
      Gown, A.M., et al. 1986. Am. J. Pathol. 125, 191.
      Product Information
      FormLiquid
      FormulationAscites diluted in proprietary buffer.
      Negative controlNormal mouse serum
      Positive controlSkeletal muscle tissue
      Preservative≤0.2% sodium azide
      Applications
      Application ReferencesFrozen Sections Otto, C.M., et al. 1994. Circulation 90, 844. Sensebe, L., et al. 1992. Leukemia 6, 459. Tsubura, A., et al. 1991. Acta Anat. 140, 287. Roholl, P.J., et al. 1990. Human Pathol. (Basel) 21, 1269. Immunoblotting Tsukada, T., et al. 1987. Am. J. Pathol. 126, 51. Immunocytochemistry-Rapid Immunoperoxidase Method Dabbs, D.J., et al. 1995. Acta Cytol. 39,157. Paraffin Sections Nayar, R., et al. 1999. Ann. Diagn. Pathol. 3,165. Ma, C.K., et al. 1997. Am. J. Clin. Pathol. 108, 641. Otto, C.M., et al. 1994. Circulation 90, 844. Kolodziejczyk, P., et al. 1993. Am. J. Surg. Pathol. 17, 1159. Tsubura, A., et al. 1991. Acta Anat.(Basel) 140, 287. Tsukada, T., et al. 1987. Am. J. Pathol. 126, 51.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesImmunoblotting (see application references)
      Immunocytochemistry (see application references)
      Frozen Sections (1:25-1:100, see application references)
      Paraffin Sections (1:25-1:100, heat pre-treatment required, see application references)
      Application CommentsExhibits cytoplasmic staining when used in immunohistochemistry. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenan SDS extract from human myocardium
      ImmunogenHuman
      CloneHHF-35
      HostMouse
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Highly Toxic
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CP64 0

      Documentation

      Anti-Actin (Ab-3) Mouse mAb (HHF-35) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Schmidt, R.A., et al. 1988. Am. J. Pathol. 131, 19.
      Tsukada, T., et al. 1987. Am. J. Pathol. 127, 389.
      Gown, A.M., et al. 1986. Am. J. Pathol. 125, 191.

      Brochure

      Title
      Antibody Sourcebookﾙ Sixth Edition
      Stem Cell Resource Brochure GBP
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 RFH
      ApplicationImmunoblotting (see application references)
      Immunocytochemistry (see application references)
      Frozen Sections (1:25-1:100, see application references)
      Paraffin Sections (1:25-1:100, heat pre-treatment required, see application references)
      Application Data
      Detection of actin by staining paraffin sections. Sample: Skeletal muscle. Primary antibody: Anti-Actin (Ab-3) Mouse mAb (HHF-35) (Cat. No. CP64) Detection: colorimetric (DAB).
      DescriptionMouse monoclonal antibody supplied as diluted ascites. Recognizes the ~42 kDa α and γ actin proteins.
      BackgroundActin (~42 kDa) is a major component of the cytoskeleton. Actin can be resolved on the basis of its isoelectric points into 3 distinctive components:α, β, and γ. Actin (Ab-3) recognizes actin of skeletal, cardiac, and smooth muscle cells, including α- and γ-isotypes of all muscle groups. It does not react with other mesenchymal cells except for myoepithelium. It also does not react with vascular endothelial cells, fibroblasts, or connective tissue. Neoplastic cells of non-muscle sarcomas, such as carcinomas, melanomas, and lymphomas, are also negative. This antibody is especially useful for the distinction between leiomyosarcoma and other spindle cell neoplasms, and in particular, the identification of rhabdomyoblasts.
      HostMouse
      Immunogen speciesHuman
      Immunogenan SDS extract from human myocardium
      CloneHHF-35
      IsotypeIgG
      Speciescanine, human, monkey, mouse, rat, shrew
      Positive controlSkeletal muscle tissue
      Negative controlNormal mouse serum
      FormLiquid
      FormulationAscites diluted in proprietary buffer.
      Preservative≤0.2% sodium azide
      CommentsExhibits cytoplasmic staining when used in immunohistochemistry. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Highly Toxic
      ReferencesSchmidt, R.A., et al. 1988. Am. J. Pathol. 131, 19.
      Tsukada, T., et al. 1987. Am. J. Pathol. 127, 389.
      Gown, A.M., et al. 1986. Am. J. Pathol. 125, 191.
      Application referencesFrozen Sections Otto, C.M., et al. 1994. Circulation 90, 844. Sensebe, L., et al. 1992. Leukemia 6, 459. Tsubura, A., et al. 1991. Acta Anat. 140, 287. Roholl, P.J., et al. 1990. Human Pathol. (Basel) 21, 1269. Immunoblotting Tsukada, T., et al. 1987. Am. J. Pathol. 126, 51. Immunocytochemistry-Rapid Immunoperoxidase Method Dabbs, D.J., et al. 1995. Acta Cytol. 39,157. Paraffin Sections Nayar, R., et al. 1999. Ann. Diagn. Pathol. 3,165. Ma, C.K., et al. 1997. Am. J. Clin. Pathol. 108, 641. Otto, C.M., et al. 1994. Circulation 90, 844. Kolodziejczyk, P., et al. 1993. Am. J. Surg. Pathol. 17, 1159. Tsubura, A., et al. 1991. Acta Anat.(Basel) 140, 287. Tsukada, T., et al. 1987. Am. J. Pathol. 126, 51.