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MABT96 Anti-Anillin Antibody, clone 5f3.1

Anti-Anillin, clone 5F3.1 is an antibody targeting the Anillin protein, validated for use in WB & ICC.

Anti-Anillin Antibody, clone 5f3.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: ANLN UniProt Number: Q9NQW6
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MABT96
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT96
      DescriptionAnti-Anillin Antibody, clone 5f3.1
      Alternate Names
      • Actin-binding protein anillin
      Background InformationAnillin is a highly conserved multidomain protein that crosslinks multiple structural and regulatory elements including, but not limited to, F-actin, active myosin II, septins, and importin. It positively regulates normal cytokinesis; however, its molecular function within this context remains unclear. Failed cytokinesis results in binucleation, which can lead to genomic instability. In human cultured cells, anillin stabilizes TCA-fixable species of RhoA at the equatorial cortex, but its exact mode of action and subsequent physiological effects have yet to be determined. In some cancers, high levels of septins are shown to out-compete importin binding, thereby promoting the retention of Anillin in the cytoplasm.
      References
      Product Information
      FormatPurified
      Control
      • Mimosine and nocodozole treated and untreated HeLa cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Anillin, clone 5F3.1 is an antibody targeting the Anillin protein, validated for use in WB & ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunocytochemistry Analysis: 1 µg/mL of this antibody detected Anillin in the contractile rings of dividing HeLa cells.
      Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:100 dilution of this antibody detected Anillin in human testis tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Anillin.
      Clone5F3.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ANLN
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~160 kDa observed. The calculated molecular weight of this protein is 124 kDa, but can be observed at ~160 kDa due to high glycosylation. Uniprot describes an isoform produced by alternative splicing at ~120 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mimosine and nocodozole treated and untreated HeLa cell lysate.
      Western Blot Analysis: 1.0 µg/mL of this antibody detected Anillin in 10 µg of mimosine and nocodozole treated and untreated HeLa cell lysate.

      Evaluated by Immunohistochemistry (Paraffin) in human testis tissue sections.
      Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:100 dilution of this antibody detected Anillin in human testis tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT96 04053252831423

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies