238702 Anti-αB-Crystallin Peptide (163-175) Rabbit pAb

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238702
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes αB-crystallin. Does not cross-react with B-crystallin or αA-crystallin.
      Catalogue Number238702
      Brand Family Calbiochem®
      Application Data
      Detection of αB-crystallin by immunoblotting. Samples: Molecular weight marker (lane 1), α crystallin (lane 2), αA crystallin (lane 3), αB crystallin (lane 4), and β crystallin (lane 5). Primary antibody: Anti-αB-Crystallin Peptide (163-175) Rabbit pAb (Cat. No. 238702) (1:1000). Detection: chemiluminescence.
      References
      ReferencesDeJong, W.W., et al. 1993. Mol. Biol. Evol. 10, 103.
      DasGupta, S., et al. 1992. Exp. Eye Res. 54, 461.
      Inaguma, Y., et al. 1992. Biochem. Biophys. Res. Commun. 182, 844.
      Horwitz, J. 1992. Proc. Natl. Acad. Sci. USA 89, 10449.
      Carver, J.A., et al. 1990. Biochem. Biophys. Acta 1204, 195.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblottting (1:2000, colorimetric)
      Application CommentsDoes not react with β-crystallin or α-A-crystallin. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic peptide (REEKPAVTAAPKK) corresponding to amino acids 163-175 of human αB-crystallin, conjugated to KLH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-αB-Crystallin Peptide (163-175) Rabbit pAb Certificates of Analysis

      TitleLot Number
      238702

      References

      Reference overview
      DeJong, W.W., et al. 1993. Mol. Biol. Evol. 10, 103.
      DasGupta, S., et al. 1992. Exp. Eye Res. 54, 461.
      Inaguma, Y., et al. 1992. Biochem. Biophys. Res. Commun. 182, 844.
      Horwitz, J. 1992. Proc. Natl. Acad. Sci. USA 89, 10449.
      Carver, J.A., et al. 1990. Biochem. Biophys. Acta 1204, 195.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-December-2008 JSW
      ApplicationImmunoblottting (1:2000, colorimetric)
      Application Data
      Detection of αB-crystallin by immunoblotting. Samples: Molecular weight marker (lane 1), α crystallin (lane 2), αA crystallin (lane 3), αB crystallin (lane 4), and β crystallin (lane 5). Primary antibody: Anti-αB-Crystallin Peptide (163-175) Rabbit pAb (Cat. No. 238702) (1:1000). Detection: chemiluminescence.
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~22 kDa α-B-crystallin protein.
      Backgroundα-crystallin is a major structural protein in the vertebrate eye lens. It occurs as large aggregates of up to 106 Da, composed of two proteins, αA- and αB-crystallin. The αA gene encodes a polypeptide of 173 residues, while αB-crystallin gene encodes a 175 residue polypeptide. αB-crystallin, like the small heat shock proteins (sHsp's), is induced by heat or osmotic shock. α-crystallin and the sHsp's protect other proteins from heat-induced aggregation. The two other crystallin families, β and γ, are homologous to each other but not to the α-family or the sHsp's.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (REEKPAVTAAPKK) corresponding to amino acids 163-175 of human αB-crystallin, conjugated to KLH
      IsotypeIgG
      Speciesbovine, human, mouse, rat
      FormLiquid
      FormulationIn PBS, 50% glycerol.
      Preservative≤0.1% sodium azide
      CommentsDoes not react with β-crystallin or α-A-crystallin. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesDeJong, W.W., et al. 1993. Mol. Biol. Evol. 10, 103.
      DasGupta, S., et al. 1992. Exp. Eye Res. 54, 461.
      Inaguma, Y., et al. 1992. Biochem. Biophys. Res. Commun. 182, 844.
      Horwitz, J. 1992. Proc. Natl. Acad. Sci. USA 89, 10449.
      Carver, J.A., et al. 1990. Biochem. Biophys. Acta 1204, 195.