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MABN2640-25UG Anti-BACE1 Antibody, clone 3D5

Anti-BACE1, clone 3D5, Cat. No. MABN2640, is a mouse monoclonal antibody that detects β-Secretase 1 (BACE1) and is tested for use in or use in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

MABN2640-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2640-25UG
      DescriptionAnti-BACE1 Antibody, clone 3D5
      Alternate Names
      • Beta-secretase 1
      • EC:3.4.23.46
      • Aspartyl protease 2
      • ASP2
      • Asp 2
      • Beta-site amyloid precursor protein cleaving enzyme 1
      • Beta-site APP cleaving enzyme 1
      • Memapsin-2
      • Membrane-associated aspartic protease 2
      Background InformationBeta-secretase 1 (UniProt: P56817; also known as EC:3.4.23.46, Aspartyl protease 2, ASP2, Asp 2, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2) is encoded by the BABACE, KIAA1149) gene (Gene ID: 23621) in human. BACE1 is a single-pass type I membrane, monomeric, glycoprotein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 22-457), a transmembrane domain (aa 458-478), and a cytoplasmic domain (aa 479-501). It also contains a DXXLL motif (aa 496-500) that is required for its proper endocytosis and retrograde transport to the trans-Golgi network, as well as for regulation of lysosomal degradation. BACE1 displays broad endopeptidase specificity and is a rate limiting enzyme that is responsible for the proteolytic processing of amyloid precursor protein (APP). It cleaves at the N-terminus of the Aβ peptide sequence, between Met671 and Asp672 of APP to yield soluble APPβ and C99. The -secretase can then cleave the C99 fragment at either Val711 or Ile713 to produce the shorter Aβ peptide (Aβ1-40) or the longer Aβ peptide (Aβ1-42). Aβ plaques are reported to induce BACE1 activity in surrounding neurons at early stages of Alzheimer s disease before neuronal death occurs. BACE1 undergoes acetylation at multiple lysine residues (126, 275, 279, 285, 299, 300, and 307) that regulates its maturation, transport to plasma membrane, and contributes to its stability. It also undergoes phosphorylation at serine 498, which is required for its interaction with GGA1 and retrograded transport from endosomal compartment to the trans-Golgi network. Six isoforms of BACE1 have been described that are produced by alternative splicing. (Ref.: Sadleir, KR., et al. (2013). Curr Alzheimer Res. 10(8); 785-789; Zhao, J., et all (2007). J. Neurosci. 27(14); :3639 -3649; Constantini, C., et al. (2007). Biochem. J. 407(3); 383-395).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-BACE1, clone 3D5, Cat. No. MABN2640, is a mouse monoclonal antibody that detects β-Secretase 1 (BACE1) and is tested for use in or use in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • ELISA
      • Immunofluorescence
      • Immunocytochemistry
      Application NotesTested Applications

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected BACE1 in NIH/3T3 cell lysate.

      Immunohistochemistry Applications: A representative lot detected BACE1 in Immunohistochemistry applications (Zhao, J., et al. (2007). J Neurosci. 27(14):3639-49).

      Immunofluorescence Analysis: A representative lot detected BACE1 in Immunofluorescence applications (Zhao, J., et al. (2007). J Neurosci. 27(14):3639-49).

      Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected BACE1 in HeLa cells.

      ELISA Analysis: A representative lot detected BACE1 in ELISA applications (Zhao, J., et al. (2007). J Neurosci. 27(14):3639-49).

      Western Blotting Analysis: A representative lot detected BACE1 in Western Blotting applications (Zhao, J., et al. (2007). J Neurosci. 27(14):3639-49; Sadleir, K.R., et al. (2013). Curr Alzheimer Res. 10(8):785--9; Pigoni, M. et al. (2020). EMBO J. 39(15):e103457).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 415 amino acids from the internal region of human β-secretase 1 (BACE1).
      EpitopeInternal
      Clone3D5
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 3D5 is a mouse monoclonal antibody that detects β-secretase 1 (BACE1).
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BACE1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~57 kDa observed; 55.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SH-SY5Y cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected BACE1 in SH-SY5Y cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2640-25UG 04065270451736