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AP1050 Anti-BMI1 (1-326) Rabbit pAb

Anti-BMI1 (1-326) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
AP1050
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~44 kDa BMI protein in U2OS cells.
      Catalogue NumberAP1050
      Brand Family Calbiochem®
      Application Data
      Detection of human BMI1 by immunoblotting. Sample: Whole cell lysate (25 µg) from U2OS cells. Primary antibody: Anti-BMI1 (1-326) Rabbit pAb (Cat. No. AP1050) (1:1000). Detection: chemiluminescence.
      References
      ReferencesLiu, L., et al. 2006. Oncogene, 45, 4370.
      Mihara, K., et al. 2006. Blood 107, 305.
      Shakhova,O., et al. 2005. J. Mol. Med. 83, 596.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Positive controlU2OS cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenfull-length, recombinant, human BMI-1
      ImmunogenHuman
      HostRabbit
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1050 0

      Documentation

      References

      Reference overview
      Liu, L., et al. 2006. Oncogene, 45, 4370.
      Mihara, K., et al. 2006. Blood 107, 305.
      Shakhova,O., et al. 2005. J. Mol. Med. 83, 596.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-August-2007 RFH
      ApplicationImmunoblotting (1:1000)
      Application Data
      Detection of human BMI1 by immunoblotting. Sample: Whole cell lysate (25 µg) from U2OS cells. Primary antibody: Anti-BMI1 (1-326) Rabbit pAb (Cat. No. AP1050) (1:1000). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~44 kDa BMI1 protein.
      BackgroundBMI1 (accession no. P35226) is a ~44 kDa nuclear protein that is a member of the polycomb group proteins (PcG). BMI1 regulates self-renewal and proliferation of hematopoietic and neural stem cells partially through modulation of H2A ubiquitination, which leads to regulation of homeobox (Hox) gene expression. BMI1 has also been reported to play a role in cancer development. It is overexpressed in high-grade B-cell non-Hodgkin lymphomas, breast cancer, non small cell lung cancer, and colorectal cancer. Furthermore, downregulation of BMI1 in cancer cells leads to an upregulation of INK4A-ARF and an increase in apoptosis and delayed cell cycle progression. BMI1 is a potential target for cancer therapy, as well as a prognostic marker for myelodysplastic syndrome.
      HostRabbit
      Immunogen speciesHuman
      Immunogenfull-length, recombinant, human BMI-1
      Specieshuman
      Positive controlU2OS cells
      FormLiquid
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesLiu, L., et al. 2006. Oncogene, 45, 4370.
      Mihara, K., et al. 2006. Blood 107, 305.
      Shakhova,O., et al. 2005. J. Mol. Med. 83, 596.