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05-1321 Anti-BMI1 Antibody, clone AF27

Anti-BMI1 Antibody, clone AF27 is a mouse monoclonal antibody for detection of BMI1 also known as BMI1 polycomb ring finger oncogene, murine leukemia viral (bmi-1) oncogene homolog & has been validated in IP, ChIP.

Anti-BMI1 Antibody, clone AF27 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: MGC12685, PCGF4, RNF51, flvi-2/bmi-1 UniProt Number: P35226
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05-1321
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, ChIPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1321
      DescriptionAnti-BMI1 Antibody, clone AF27
      Alternate Names
      • B lymphoma Mo-MLV insertion region 1 homolog
      • B lymphoma Mo-MLV insertion region 1 homolog (mouse)
      • BMI1 polycomb ring finger oncogene
      • Polycomb group RING finger protein 4
      • RING finger protein 51
      • murine leukemia viral (bmi-1) oncogene homolog
      • oncogene BMI-1
      • polycomb group protein Bmi1
      • polycomb group ring finger 4
      Background InformationThe BMI1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors. Several lines of evidence implicate BMI1 in tumorigenesis. BMI1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. BMI1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene, leads to neoplastic transformation. The BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. The transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires BMI1.
      References
      Product Information
      FormatPurified
      Control
      • MEF1 cell lysate
      PresentationPurified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-BMI1 Antibody, clone AF27 is a mouse monoclonal antibody for detection of BMI1 also known as BMI1 polycomb ring finger oncogene, murine leukemia viral (bmi-1) oncogene homolog & has been validated in IP, ChIP.
      Key Applications
      • Immunoprecipitation
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation (ChIP):
      A previous lot was used by an independent laboratory in ChIP. (Bracken, A.P., et al., 2007).
      Biological Information
      ImmunogenMBP-tagged recombinant protein corres-ponding to full length human BMI1.
      CloneAF27
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes BMI1.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MGC12685
      • PCGF4
      • RNF51
      • flvi-2/bmi-1
      Purification MethodAnti-BMI1, clone AF27
      UniProt Number
      UniProt SummaryFUNCTION: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
      SUBUNIT STRUCTURE: Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Also component of E3 ubiquitin ligase complex or human polycomb repressive complex 1-like at least composed of PHC2, BMI1, RING1 and RNF2. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1.
      SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
      PTM: May be polyubiquitinated; which does not lead to proteasomal degradation.
      INVOLVEMENT IN DISEASE: Cooperates with the MYC oncogene to produce B-lymphomas.
      SEQUENCE SIMILARITIES: Contains 1 RING-type zinc finger.
      Molecular Weight~35 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunoprecipitation in MEF1 cell lysate.
      Immunoprecipitation Analysis: 10 μg/mL of this antibody immunoprecipitated BMI1 from 100 μg of MEF1 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at 2-8°C for 1 year from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-1321 04053252408649