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ST1695 Anti-BOLL Mouse mAb (5B8)

Anti-BOLL Mouse mAb (5B8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Boule-like

ST1695
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~35-38 kDa BOLL protein in SW13 cells and human liver tissue.
      Catalogue NumberST1695
      Brand Family Calbiochem®
      SynonymsAnti-Boule-like
      Application Data
      Detection of human BOLL by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-BOLL Mouse mAb (5B8) (Cat. No. ST1695) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human BOLL by immunoblotting. Sample: Whole cell lysate from SW13 cells. Primary antibody: Anti-BOLL Mouse mAb (5B8) (Cat. No. ST1695) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Positive controlHuman liver tissue, SW13 cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 185-284 human BOLL, expressed as a GST fusion protein
      Clone5B8
      HostMouse
      IsotypeIgG2a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1695 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-November-2010 JLN
      SynonymsAnti-Boule-like
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human BOLL by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-BOLL Mouse mAb (5B8) (Cat. No. ST1695) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human BOLL by immunoblotting. Sample: Whole cell lysate from SW13 cells. Primary antibody: Anti-BOLL Mouse mAb (5B8) (Cat. No. ST1695) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~35-38 kDa BOLL protein.
      BackgroundThis gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 185-284 human BOLL, expressed as a GST fusion protein
      Clone5B8
      IsotypeIgG2a
      Specieshuman
      Positive controlHuman liver tissue, SW13 cells
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review