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ABE1966 Anti-BPTF (VCU19)

Anti-BPTF (VCU19) Antibody, Cat. No. ABE1966, is a highly specific rabbit polyclonal antibody that targets BPTF and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq and Western Blotting applications.

Anti-BPTF (VCU19) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Bptf, Fac1, Falz UniProt Number: A2A654, E9Q6A7
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ABE1966
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MChIP, WB, ChIP-seqRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1966
      DescriptionAnti-BPTF (VCU19)
      Alternate Names
      • Nucleosome-remodeling factor subunit BPTF
      • Bromodomain and PHD finger-containing transcription factor
      • Fetal Alz-50 clone 1 protein
      • Fetal Alzheimer antigen
      Background InformationNucleosome-remodeling factor subunit BPTF (UniProt A2A654 and E9Q6A7; also known as Bromodomain and PHD finger-containing transcription factor, Fetal Alz-50 clone 1 protein, Fetal Alzheimer antigen) is encoded by the Bptf (also known as Fac1, Falz) gene (Gene ID 207165) in murine species. BPTF, SNF2L and pRBAP46/48 are the three subunits of the nucleosome-remodelling factor NURF, an ISWI chromatin-remodeling complex that catalyzes ATP-dependent nucleosome sliding. BPTF mediates NURF target site specificity via interactions with transcription factors, histone variants and histone modifications on transcriptionally active genes. BPTF plays an important role in regulating nucleosome occupancy at nucleosome-free regions (NFRs) at sites occupied by the multivalent factors CTCF and cohesin via direct interaction with CTCF and the cohesin subunit SA2. In addition, BPTF also interacts with c-MYC and plays an essential role in its genomic distribution and biological activity. BPTF is necessary for the survival of c-MYC-overexpressing cells and for c-MYC-driven tumorigenesis in the mouse pancreas. BPTF knockdown leads to decreased c-MYC chromatin accessibility and significantly delays tumour development in pre-neoplastic pancreatic acinar cells. Consistently, BPTF expression in human tumours positively correlates with activation of c-MYC gene signatures.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-BPTF (VCU19) Antibody, Cat. No. ABE1966, is a highly specific rabbit polyclonal antibody that targets BPTF and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq and Western Blotting applications.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Western Blotting
      • ChIP-seq
      Application NotesChromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected BPTF chromatin occupancy at known BPTF target sites by ChIP-PCR using mouse embryonic stem cell (mESC) chromatin preparation (Courtesy of Joseph Landry, Ph.D, Virginia Commonwealth University School of Medicine).

      ChIP-seq Analysis: A representative lot mapped BPTF chomsome 19 enrichment sites by ChIP-seq analysis (Courtesy of Joseph Landry, Ph.D, Virginia Commonwealth University School of Medicine).

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected BPTF occupancy at Ctcf-targeted chromatin sites containing BPTF-dependent nucleosome-free regions (NFRs) using mouse embryonic stem cell (mESC) chromatin preparation (Qiu, Z., et al. (2015). Mol. Cell. Biol. 35(1):224-237).

      Western Blotting Analysis: A 1:1,250 dilution from a representative lot detected BPTF in 50 µg of wild-type, but not BPTF-knockout, mouse embryonic fibroblast (MEF) lysate (Courtesy of Joseph Landry, Ph.D, Virginia Commonwealth University School of Medicine).
      Biological Information
      ImmunogenMBP-tagged recombinant mouse BPTF fragment from the region between the third and fourth coiled coil.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificitySpecificity of this polyclonal antibody was confirmed by target band detection in wild-type, but not BPTF-knockout, mouse embryonic fibroblast (MEF) lysate (Courtesy of Joseph Landry, Ph.D, Virginia Commonwealth University School of Medicine).
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Rat based on 100% sequence homology. Also expected to react with Human based on high sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Bptf
      • Fac1
      • Falz
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~300 kDa observed. 333.2/321.6 kDa (mouse; UniProt A2A654/E9Q6A7) and 338.3/325.1/322.2 kDa (human isoform 1/2/3; UniProt Q12830) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected BPTF in 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1966 04054839057908