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MABC1176 Anti-Bax Antibody, clone 6A7

Anti-Bax, clone 6A7, Cat. No. MABC1176, is a mouse monoclonal antibody that detects Bax and has been tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Anti-Bax Antibody, clone 6A7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: BAX, BCL2L4 UniProt Number: Q07812
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MABC1176
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIF, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1176
      DescriptionAnti-Bax Antibody, clone 6A7
      Alternate Names
      • Apoptosis regulator BAX
      • Bcl-2-like protein 4
      • Bcl2-L-4
      Background InformationApoptosis regulator Bax (UniProt: Q07812; also known as Bcl-2-like protein 4, Bcl2-L-4) is encoded by the BAX (also known as BCL2L4) gene (Gene ID: 581) in human. Bax is a pro-apoptotic member of the Bcl-2 family of proteins. It displays extensive homology with Bcl-2 and is variably expressed in different cells, usually at very low concentrations in normal cells. Upon induction of apoptosis Bax undergoes a conformational change and translocates from cytosol to mitochondrial membrane. Bax forms homodimers as well as heterodimers with Bcl-2 through common binding motif and the ratio of Bax to Bcl-2 determines the survival or death of cells during apoptosis. BAX is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. Clone 6A7 reacts with multiple isoforms of Bax.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Bax, clone 6A7, Cat. No. MABC1176, is a mouse monoclonal antibody that detects Bax and has been tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Bax in Western Blotting applications (Xin, M., et. al. (2014). Nat Commun. 5:4935).

      Immunofluorescence Analysis: A representative lot detected Bax in Immunofluorescence applications (Xin, M., et. al. (2014). Nat Commun. 5:4935).

      Immunoprecipitation Analysis:A representative lot detected Bax in Immunoprecipitation applications (Xin, M., et. al. (2014). Nat Commun. 5:4935; Hsu, Y.T., et. al. (1998). J Biol Chem. 273(17):10777-83).
      Biological Information
      ImmunogenKLH-cojugated linear peptide coprresponding to 13 amino acids from the N-terminal region of human BAX.
      Clone6A7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6A7 recognizes BAX in human cells. It targets an epitope with in 13 amino acids from the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse and Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BAX
      • BCL2L4
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~15 kDa observed; 21.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s). Multiple isoforms for this target (8), ranging in MWs between 4-24 kDa, are reported. Most of the predicted MWs reside between 12-19 kDa. Based on the sequence it is expected to react with isoforms 1,2,4,5, and 8.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunoprecipitation in THP-1 cell lysates.

      Immunoprecipitation Analysis: A 1:250 dilution of this antibody detected Bax in THP-1 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1176 04054839322495