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AB1735 Anti-Bid Antibody

Detect Bid using this Anti-Bid Antibody validated for use in WB, IP, IH.

Anti-Bid Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: BCL2 & Inhibition Gene Symbol: BID, FP497, MGC42355, MGC15319 UniProt Number: P55957
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AB1735
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, RbWB, IP, IHCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB1735
      Replaces04-438
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Bid Antibody
      Background InformationBid, a BH3 domain-containing proapoptotic Bcl-2 family member, is known to interact with Bcl-2 and Bax. It has been reported that Bid induces cytochrome c release from mitochondria following cleavage by caspase-8 in response to apoptotic stimuli transmitted through cell surface death receptors. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
      References
      Product Information
      FormatSerum
      PresentationWhole serum without preservatives
      Quality LevelMQ100
      Applications
      ApplicationDetect Bid using this Anti-Bid Antibody validated for use in WB, IP, IH.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry
      Application NotesWestern blot: Recommended antibody dilution 1:3,000 to 1:6,000. Recognizes a band migrating at approximately 22 kDa corresponding to Bid.

      Immunoprecipitation: Recommended antibody dilution 1:200

      Immunohistochemistry: Recommended antibody dilution 1:1500

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant full length GST-tagged mouse Bid protein
      HostRabbit
      SpecificityAB1735 recognizes Bid from human, mouse, rat and rabbit.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rabbit
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
      Gene Symbol
      • BID
      • FP497
      • MGC42355
      • MGC15319
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P55957 # The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
      SIZE: 195 amino acids; 21995 Da
      SUBUNIT: Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2 (By similarity).
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Mitochondrion membrane (By similarity). Note=When uncleaved, it is predominantly cytoplasmic. & BH3-interacting domain death agonist p15: Mitochondrion membrane (By similarity). Note=Translocates to mitochondria as an integral membrane protein (By similarity). & BH3-interacting domain death agonist p13: Mitochondrion membrane (By similarity). Note=Associated with the mitochondrial membrane (By similarity). & Isoform 1: Cytoplasm. & Isoform 3: Cytoplasm. & Isoform 2: Mitochondrion membrane. Note=A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively.
      TISSUE SPECIFICITY: Isoforms 2 and 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
      DOMAIN: SwissProt: P55957 Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
      PTM: TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products (By similarity). & Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY:
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C for up to 12 months.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB1735 04053252742422