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MABS2321-100UG Anti-Biotin Antibody, clone 8H6.8

Anti-Biotin, clone 8H6.8, Cat. No. MABS2321, is a mouse monoclonal antibody that detects Biotin-conjugated proteins and is tested for use in ELISA, Immunofluorescence, and Western Blotting.

MABS2321-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2321-100UG
      DescriptionAnti-Biotin Antibody, clone 8H6.8
      Alternate Names
      • Vitamin B7
      Background InformationBiotin, a bicyclic compound, is a water-soluble vitamin with a ureido (N-CO-N) and a tetrahydrothiophene ring. The ureido group is involved in its binding to avidin. The tetrahydrothiophene group is a valeric acid moiety. Although biotin molecule can exist in eight stereoisomers, the D-biotin isomer is the only one with biological activity. It plays important roles in a variety of critical metabolic reactions in the cell, and thus, is essential for normal human health, growth, and development. Its deficiency, particularly during pregnancy, leads to embryonic growth retardation, congenital malformation, and death. In mammals, biotin serves as a cofactor for four carboxylases that catalyze the transfer of a carboxyl group to targeted substrates. The four biotin-dependent carboxylases are: pyruvate carboxylase; propionly-CoA carboxylase; β-methylcrotonyl-CoA carboxylase; and acetyl-CoA carboxylase. These carboxylases play a critical role in the intermediate metabolism of gluconeogenesis, fatty acid synthesis, and amino acid catabolism. It is also reported to play a role in normal immune functions, including the production of antibodies, normal macrophage function, and the differentiation of T and B lymphocytes. This monoclonal antibody recognizes biotin conjugated to various proteins in ELISA and other immunochemical applications. (Ref.: Said, SM. (2012). Subcell. Biochem. 56; 1-19; Rabin, BS. (1983). J. Nutr. 113(11); 2316 2322).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Biotin, clone 8H6.8, Cat. No. MABS2321, is a mouse monoclonal antibody that detects Biotin-conjugated proteins and is tested for use in ELISA, Immunofluorescence, and Western Blotting.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Western Blotting
      Application NotesTested Applications

      Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected Biotin-BSA.

      Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected Biotin-conjugates in Human kidney tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenBiotin conjugated to KLH.
      EpitopeUnknown
      Clone8H6.8
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 8H6.8 is a mouse monoclonal antibody that detects biotin-conjugated proteins.
      IsotypeIgG1κ
      Species Reactivity
      • All
      Species Reactivity Note. Predicted to react with All based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight244.31 Da calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by ELISA with Biotin-BSA.

      ELISA Analysis: Serial dilutions from a representative lot detected Biotin-BSA in ELISA application.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2321-100UG 04065271175020