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MABN2296-25UG Anti-C9orf72 peptide 4 Antibody, clone 12E7

Anti-C9orf72 peptide 4, clone 12E7, Cat. No. MABN2296, is a rat monoclonal antibody that detects C9orf72 and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

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MABN2296-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2296-25UG
      DescriptionAnti-C9orf72 peptide 4 Antibody, clone 12E7
      Alternate Names
      • Guanine nucleotide exchange C9orf72
      Background InformationGuanine nucleotide exchange C9orf72 (UniProt: Q96LT7; also known as C9orf72) is encoded by the C9orf72 gene (Gene ID: 203228) in human. C9orf72 generates three transcripts through alternative splicing that encode 2 protein isoforms: a long isoform of about 54 kDa (termed C9-L), corresponding to variants 2 and 3, and a short isoform of about 24 kDa (termed C9-S) corresponding to variant 1. Both isoforms are widely expressed, including in kidney, lung, liver, heart, testis, and several brain regions. The long isoform of 481 amino acids is shown to be the predominant form that is expressed in human brain. Expansion of a GGGGCC (G4C2) hexanucleotide repeat sequence in the non-coding region of human C9orf72 is the most common genetic abnormality in familial and sporadic frontotemporal dementia (FTD) and motor neuron disease (MND), with amyotrophic lateral sclerosis (ALS) as the most frequent form. Both, C9-L and C9-S are reported to display distinct subcellular localizations in cerebellar Purkinje cells of both c9-ALS and non c9-ALS cases. C9-L is shown to be spread diffusely throughout the cytoplasm, whereas C9- S is localized to the nuclear membrane of Purkinje cells of both c9-ALS and non-c9-ALS cases. Also, C9-L is shown to be present mainly in the high-salt Triton and urea-soluble fractions, whereas C9-S is shown to be abundant in both the low- and high-salt Triton fractions, indicating differences in their subcellular localizations. (Ref.: Frick, P., et al. (2018). Acta Neuropathol. Commun. 6(1); 72).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-C9orf72 peptide 4, clone 12E7, Cat. No. MABN2296, is a rat monoclonal antibody that detects C9orf72 and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • ELISA
      • Immunofluorescence
      • Immunocytochemistry
      Application NotesTested Applications

      Immunohistochemistry Applications: A representative lot detected C9orf72 in Immunohistochemistry applications (Frick, P., et al. (2018). Acta Neuropathol Commun. 6(1):72).

      Western Blotting Analysis: A representative lot detected C9orf72 in Western Blotting applications (Frick, P., et al. (2018). Acta Neuropathol Commun. 6(1):72).

      Immunofluorescence Analysis: A representative lot detected C9orf72 peptide 4 in Immunofluorescence applications (Frick, P., et al. (2018). Acta Neuropathol Commun. 6(1):72).

      Immunocytochemistry Analysis: A representative lot detected C9orf72 peptide 4 in Immunocytochemistry applications (Frick, P., et al. (2018). Acta Neuropathol Commun. 6(1):72).

      ELISA Analysis: A representative lot detected C9orf72 peptide 4 in ELISA applications (Frick, P., et al. (2018). Acta Neuropathol Commun. 6(1):72).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of human Guanine nucleotide exchange C9orf72.
      EpitopeN-terminal half
      Clone12E7
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone 12E7 is a rat monoclonal antibody that detects Guanine nucleotide exchange C9orf72. It targets an epitope within 16 amino acids from the N-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman. Mouse. Predicted to react with Monkey based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • C9orf72
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~54 kDa observed; 54.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: A 1:250 dilution of this antibody detected C9orf72 in HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2296-25UG 04065267780504