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AP1156 Anti-CCL3 Mouse mAb (4E7)

AP1156
  
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      Overview

      Replacement Information

      Key Spec Table

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      Description
      Overview

      This product has been discontinued.





      Recognizes the CCL3 protein.
      Catalogue NumberAP1156
      Brand Family Calbiochem®
      SynonymsAnti-Chemokine (C-C) Ligand 3, anti-MIP-1-α
      Application Data
      Detection of human CCL3 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with partial, recombinant, human CCL3 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-CCL3 Mouse mAb (4E7) (Cat. No. AP1156) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 24-93 of human CCL3, expressed as a GST fusion protein
      Clone4E7
      HostMouse
      IsotypeIgG2a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-October-2010 RFH
      SynonymsAnti-Chemokine (C-C) Ligand 3, anti-MIP-1-α
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human CCL3 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with partial, recombinant, human CCL3 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-CCL3 Mouse mAb (4E7) (Cat. No. AP1156) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the CCL3 protein.
      BackgroundCCL3, also known as Macrophage inflammatory protein-1α (MIP-1α), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocyte. This protein plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 24-93 of human CCL3, expressed as a GST fusion protein
      Clone4E7
      IsotypeIgG2a
      Specieshuman
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review