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MABT333 Anti-CD167a/DDR1 Antibody, clone 5D5

This mouse monoclonal Anti-CD167a/DDR1, clone 5D5 Antibody, Cat. No. MABT333 is validated for use in ELISA, Flow Cytometry, Immunocytochemistry, and Neutralization applications.

Anti-CD167a/DDR1 Antibody, clone 5D5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: DDR1, CAK, EDDR1, MCK10, NEP, NTRK4, PTK3A, RTK6, TRKE UniProt Number: Q08345
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MABT333
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT333
      DescriptionAnti-CD167a/DDR1 Antibody, clone 5D5
      Alternate Names
      • Epithelial discoidin domain-containing receptor 1
      • CD167 antigen-like family member A
      • CD167a
      • Cell adhesion kinase
      • Discoidin receptor tyrosine kinase
      • Epithelial discoidin domain receptor 1
      • HGK2
      • Mammary carcinoma kinase 10
      • MCK-10
      • Protein-tyrosine kinase 3A
      • Protein-tyrosine kinase
      • RTK-6
      • TRK E
      • Tyrosine kinase DDR
      • Tyrosine-protein kinase CAK
      Background InformationEpithelial discoidin domain-containing receptor 1 (EC 2.7.10.1; UniProt Q08345; also known as CD167 antigen-like family member A, CD167a, Cell adhesion kinase, Discoidin receptor tyrosine kinase, Epithelial discoidin domain receptor 1, HGK2, Mammary carcinoma kinase 10, MCK-10, Protein-tyrosine kinase 3A, Protein-tyrosine kinase RTK-6, TRK E, Tyrosine kinase DDR, Tyrosine-protein kinase CAK) is encoded by the DDR1 (also known as CAK, EDDR1, MCK10, NEP, NTRK4, PTK3A, RTK6, TRKE) gene (Gene ID 780) in human. Discoidin domain-containing receptors (DDRs) belong to the superfamily of transmembrane receptor tyrosine kinases (RTKs). DDRs are distinguished from other RTKs by their extracellular discoidin motif and their utilization of collagens as internal ligands. While DDR1 binds essentially all types of collagens, DDR2 shows a preference for type I, II and III fibrillar collagens, and nonfibrillar type X collagen. DDR1 is widely expressed in epithelial cells in lung, kidney, colon, and brain, whereas DDR2 is primarily expressed in mesenchymal cells including fibroblasts, myofibroblasts, smooth muscle cells, and chondrocytes in kidney, skin, lung, heart, and connective tissues. DDRs are important mediators of fundamental cellular processes, such as proliferation, survival, differentiation, adhesion and matrix remodeling, their dysregulations are reported to cause a number of human diseases, including fibrotic disorders, atherosclerosis and cancer. DDR1 is initially produced with a signal peptide sequence (a.a. 1-20), the removal of which yields the mature protein with an extracellular region (a.a. 21-417) that contains the Discoidin domain (F5/8 type C domain or C2-like domain) at the N-terminal half (a.a. 31-185) and a collagen-binding site within the membrane-distal DS domain (a.a. 192-367), followed by a transmembrane segment (a.a. 418-438) and a cytoplasmic region (a.a. 439-913) that harbors a WW domain-interacting PPxY motif (a.a. 481-484) and the kinase domain (a.a. 610-905). Alternative splicings produce five isoforms, isoforms 2 (CAK II, DDR1a, Short) and 5 are expressed with a truncated cytoplasmic domain, while isoform 3 (DDR1d) is expressed in a 243-a.a. soluble form and isoform 4 is expressed with a 6-a.a. insertion (SFSLFS) between Ala665 and Arg666 of the canonical isoform 1 (CAK I, DDR1b) sequence.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1 in PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-CD167a/DDR1, clone 5D5 Antibody, Cat. No. MABT333 is validated for use in ELISA, Flow Cytometry, Immunocytochemistry, and Neutralization applications.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 10 µg/mL from a representative lot detect detected CD167a/DDR1 on the surface of 3% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized human neonatal foreskin keratinocytes (Courtesy of Birgit Leitinger, Ph.D., Imperial College, London, United Kingdom).

      ELISA Analysis: A representative lot bound immobilized recombinant human DDR1b extracellular domain (Kd ~2 nM) without affinity toward DDR2 extracellular domain or DDR1b membrane-distal DS domain containing the collagen-binding site (Carafoli, F., et al. (2012). Structure. 20(4):688-697).

      Flow Cytometry Analysis: A representative lot detected the exogenously expressed DDR1b wild-type and mutant constructs on the surface of transfected HEK293 cells (Carafoli, F., et al. (2012). Structure. 20(4):688-697).

      Neutralizing Analysis: A representative lot inhibited collagen-induced autophosphorylation of DDR1b exogenously expressed on the surface of transfected HEK293 cells (Carafoli, F., et al. (2012). Structure. 20(4):688-697).
      Biological Information
      ImmunogenRecombinant human DDR1b extracellular domain.
      Epitopeextracellular domain
      Clone5D5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5D5 bound recombinant human DDR1b extracellular domain without affinity toward DDR2 extracellular domain or DDR1b membrane-distal DS domain containing the collagen-binding site (Carafoli, F., et al. (2012). Structure. 20(4):688-697). Clone 5D5 does not recognize denatured DDR1b and therefore is not suitable for Western blotting application.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DDR1
      • CAK
      • EDDR1
      • MCK10
      • NEP
      • NTRK4
      • PTK3A
      • RTK6
      • TRKE
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight99.18/101.1 kDa (mature/pro-isoform 1, CAK I, DDR1b), 95.22/97.17 kDa (mature/pro-isoform 2, CAK II, DDR1a, Short), 25.18/27.13 kDa (mature/pro-isoform 3, DDR1d), 99.84/101.8 kDa (mature/pro-isoform 4), 97.11/99.06 kDa (mature/pro-isoform 5) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in T47D human breast cancer cells.

      Flow Cytometry Analysis: 0.1 µg of this antibody detected CD167a/DDR1 on the surface of one million 2% paraformaldehyde-fixed T47D human breast cancer cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT333 04054839055607

      Documentation

      Anti-CD167a/DDR1 Antibody, clone 5D5 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CD167a/DDR1 Antibody, clone 5D5 Certificates of Analysis

      TitleLot Number
      Anti-CD167a/DDR1, clone 5D5 - 3244392 3244392
      Anti-CD167a/DDR1, clone 5D5 - 3841518 3841518
      Anti-CD167a/DDR1, clone 5D5 - 3884757 3884757
      Anti-CD167a/DDR1, clone 5D5 - 3953188 3953188
      Anti-CD167a/DDR1, clone 5D5 - 4063582 4063582
      Anti-CD167a/DDR1, clone 5D5 - 4136847 4136847
      Anti-CD167a/DDR1, clone 5D5 -Q2737524 Q2737524
      Anti-CD167a/DDR1, clone 5D5 Monoclonal Antibody - 3927987 3927987