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MABS1927 Anti-CD173 and CD174 Antibody, clone BRIC 231

Anti-CD173 CD174, clone BRIC 231, Cat. No. MABS1927, is a mouse monoclonal antibody that detects CD173/CD174 in human and is tested for use in Flow cytometry and Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling UniProt Number: P22083
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MABS1927
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, FCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1927
      DescriptionAnti-CD173 and CD174 Antibody, clone BRIC 231
      Alternate Names
      • Fucosylated histo-blood group antigens H type 2/Lewis Y
      • Clone BRIC 231
      Background InformationCD173 (also known as fucosylated histo-blood group antigens H type 2; Blood group O) and CD174 (also known as Lewis Y or LeY) are developmentally regulated cell surface carbohydrate antigens that are expressed to varying degrees on many human carcinomas. They are also expressed on CD34+ hematopoietic progenitor cells, but are absent on mature lymphocytes. Hence, it is suggested that CDS173 and CD174 play a role in homing process of hematopoietic stem cells. Fucosylated glycans are known to be involved in cell-cell and cell-matrix interactions, differentiation, cell proliferation, and apoptosis. CD173 is a monofucosylated H2 where as CD174 is a difucosylated LeY. They are structurally related and have 2 precursor chains (Galbeta1-4GlcNAc-) in common. CD173 is synthesized through the addition of a fucose on type 2 precursor chains by the alpha 1-2 fucosyltransferases. CD174 results from the epistatic interaction on type 2 precursor chains, of the products of the alpha 1-2 fucosyltransferases and alpha 1-3 fucosyltransferases.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CD173 CD174, clone BRIC 231, Cat. No. MABS1927, is a mouse monoclonal antibody that detects CD173/CD174 in human and is tested for use in Flow cytometry and Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      • Flow Cytometry
      Application NotesImmunocytochemistry Analysis: A representative lot detected CD173 CD174 in Immunocytochemistry applications (Cao, Y., et. al. (2001). Glycobiology. 11(8):677-83).

      Flow Cytometry Analysis: A representative lot detected CD173 CD174 in Flow Cytometry applications (Cao, Y., et. al. (2001). Glycobiology. 11(8):677-83).
      Biological Information
      ImmunogenWhole HEL cells
      CloneBRIC 231
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone BRIC231 detects CD173/CD174 in human cells.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight59.08 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in KG1 cells.

      Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected CD173 CD174 in KG1 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1927 04054839204210