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MABS2273-25UG Anti-CD200/HPi1 Antibody, clone HIC0-4F9

Anti-CD200/HPi1, clone HIC0-4F9, Cat. No. MABS2273, is a mouse monoclonal antibody that detects CD200/HPi1 and is tested for use in and Flow Cytometry and Immunohistochemistry.

MABS2273-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2273-25UG
      DescriptionAnti-CD200/HPi1 Antibody, clone HIC0-4F9
      Alternate Names
      • OX-2 membrane glycoprotein
      Background InformationOX-2 membrane glycoprotein (UniProt: P41217; also known as CD200) is encoded by the CD200 (also known as MOX1, MOX2, My033) gene (Gene ID: 4345) in human. CD200 is a widely expressed, single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 31-232), a transmembrane domain (aa 233-359), and a cytoplasmic domain (aa 260-278). It also contains one V-type Ig-like domain (aa 31-141) and one C2-type Ig-like domain (aa 142-232). It is widely expressed on a broad range of cells including lymphoid cells, neurons, and endothelium. It interacts with CD200R, an immunoglobulin superfamily inhibitory receptor that is expressed primarily on myeloid/monocyte lineage cells and has a suppressive effect on T cell-mediated immune response. CD200 also serves as a pani-slet marker of pancreatic endocrine cells, including insulin-expressing β- cells, but does not recognize exocrine cells. Its levels are reported to be elevated in pancreatic β cells following SARS-CoV-2 infection and this infection is strictly dependent on ACE2 receptor. Three isoforms of CD200 have been described that are produced by alternative splicing. (Ref.: van der Heide, V., et al. (2022). Cell Rep. 38(11); 110508; Dorfman, DM., and Shahsafaei, A. (2010). Am. J. Clin. Pathol. 134(5); 726-733; Dorrell, C., et al. (2008). Stem Cell Res. 1(3); 183-194).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-CD200/HPi1, clone HIC0-4F9, Cat. No. MABS2273, is a mouse monoclonal antibody that detects CD200/HPi1 and is tested for use in and Flow Cytometry and Immunohistochemistry.
      Key Applications
      • Immunohistochemistry
      • Flow Cytometry
      Application NotesTested Applications

      Immunohistochemistry Applications: A representative lot detected CD200/HPi1 in Immunohistochemistry applications in fresh-frozen normal pancreas tissues (Courtesy of Craig Dorrell, Ph.D., Oregon Health & Science University, Portland, Oregon USA).

      Immunohistochemistry Applications: A representative lot detected CD200/HPi1 in Immunohistochemistry applications (Dorrell, C., et al. (2008). Stem Cell Res. 1(3):183-94).

      Flow Cytometry Analysis: A representative lot detected CD200/HPi1 in Flow Cytometry applications (van der Heide, V., et al. (2022). Cell Rep. 38(11):110508).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenHuman pancreatic enriched islet cells.
      EpitopeUnknown
      CloneHIC0-4F9
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone HIC0-4F9 is a mouse monoclonal antibody that detects CD200/HPi1 protein.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CD200
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight31.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2273-25UG 04065271051799