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MABF2105-100UG Anti-CD6 Antibody, clone UMCD6

Anti-CD6, clone UMCD6, Cat. No. MABF2105 is a mouse monoclonal antibody that detects CD6 and has been tested for use in Dot Blot, Flow Cytometry, Immunoprecipitation, Function Analysis, Inhibition assay.

Anti-CD6 Antibody, clone UMCD6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: CD6 UniProt Number: P30203
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MABF2105-100UG
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HDB, FC, IP, Function Assay, InhibitionMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2105-100UG
      DescriptionAnti-CD6 Antibody, clone UMCD6
      Alternate Names
      • T-cell differentiation antigen CD6
      • T12
      • TP120
      Background InformationT-cell differentiation antigen CD6 (UniProt: P30203; also known as T12, TP120, CD6) is encoded by the CD6 gene (Gene ID: 923) in human. CD6 is a single-pass type I membrane protein that serves as a cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166. It contributes to signaling cascades triggered by activation of the TCR/CD3 complex and functions as a costimulatory molecule to promote T-cell activation and proliferation. It is also involved in the formation and maturation of the immunological synapse. CD6 has been detected on peripheral blood T-cells and on natural killer (NK) cells and is also detected in spleen, thymus, appendix, lymph nodes. CD6 is known to bind both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria and LPS binding leads to the activation of signaling cascades and down-stream MAP kinases. It is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off in the mature form. It has an extracellular domain (aa 18-402), a transmembrane domain (aa 403-423), and a cytoplasmic domain (aa 424-668). Seven isoforms of CD6 have been reported that are produced by alternative splicing.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
      Applications
      ApplicationAnti-CD6, clone UMCD6, Cat. No. MABF2105 is a mouse monoclonal antibody that detects CD6 and has been tested for use in Dot Blot, Flow Cytometry, Immunoprecipitation, Function Analysis, Inhibition assay.
      Key Applications
      • Dot Blot
      • Flow Cytometry
      • Immunoprecipitation
      • Function Assay
      • Inhibition
      Application NotesInhibition Analysis: A representative lot inhibited antigen-specific responses to tetanus toxoid, but not to the superantigen SEA. (Singer, N.G., et. al. (1996). Immunology. 88(4):537-43).

      Induces Function: A representative lot showed strong synergistic effect with phorbol ester in inducing T cell activation and enhanced the autologous mixed lymphocyte reaction. (Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92).

      Dot Blot Analysis: A representative lot detected CD6 in Dot Blot applications (Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated CD6 in Immunoprecipitation applications (Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92).

      Flow Cytometry Analysis: A representative lot detected CD6 in Immunoprecipitation applications (Singer, N.G., et. al. (1996). Immunology. 88(4):537-43; Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92; Bott, C.M., et. al. (1994). J Immunol. 153(1):1-9; Li, Y., et. al. (2017). Proc Natl Acad Sci USA. 114(10):2687-2692).
      Biological Information
      ImmunogenST-1 T cells isolated from human knee synovium.
      CloneUMCD6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone UMCD6 specifically detects CD6 in human cells.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CD6
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight71.80 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in PMA stimulated Jurkat cells.

      Flow Cytometry Analysis: A 1:1000 dilution of this antibody detected CD6 in one million Jurkat cells stimulated with PMA.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2105-100UG 04054839531996

      Documentation

      Anti-CD6 Antibody, clone UMCD6 Certificates of Analysis

      TitleLot Number
      Anti-CD6, clone UMCD6 - 3471511 3471511
      Anti-CD6, clone UMCD6 - 3679429 3679429
      Anti-CD6, clone UMCD6 - 3714287 3714287
      Anti-CD6, clone UMCD6 - 3877639 3877639
      Anti-CD6, clone UMCD6 - 3979385 3979385
      Anti-CD6, clone UMCD6 - 3987328 3987328
      Anti-CD6, clone UMCD6 - 4009720 4009720
      Anti-CD6, clone UMCD6 - 4128021 4128021
      Anti-CD6, clone UMCD6 - 4230537 4230537
      Anti-CD6, clone UMCD6 - 4250014 4250014
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