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MABF3230-25UG Anti-CD70 Antibody, clone FR70

Anti-CD70, clone FR70, Cat. No. MABF3230, is a rat monoclonal antibody that detects mouse CD70 and is tested for use in Immunoprecipitation, Western Blotting, Flow Cytometry, and Inhibition Assay.

MABF3230-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3230-25UG
      DescriptionAnti-CD70 Antibody, clone FR70
      Alternate Names
      • CD70 antigen
      • CD27 ligand
      • Tumor necrosis factor ligand superfamily member 7
      • Cd27l
      • Cd27lg
      • Tnfsf7
      Background InformationCD70 antigen (UniProt: O55237; also known as CD27 ligand, CD27-L, Tumor necrosis factor ligand superfamily member 7) is encoded by the Cd70 (also known as Cd27l, Cd27lg, Tnfsf7) gene (Gene ID: 21948) in mouse. CD70 is a type II transmembrane protein belonging to the tumor necrosis factor (TNF) family. The extracellular domain is involved in binding to its receptor, CD27, which is a member of the TNF receptor family. CD70, together with CD27, plays a critical role in the immune system and involved in T and B cell activation and differentiation and in the formation and maintenance of memory T cells, which are essential for long-term immunity. CD70 expression is tightly regulated and primarily found on activated lymphocytes and dendritic cells. Overexpression of CD70 in tumors can lead to immune evasion by inducing T cell exhaustion and apoptosis through chronic CD70-CD27 signaling. Targeting CD70 or CD27 is being explored as a therapeutic strategy in oncology. Dysregulated CD70 expression can contribute to autoimmune conditions and chronic infections as well. (Ref.: Flieswasser, T., et al., 2022, J. Exp. Clin. Cancer Res., 41:12, Oshima, H., et al. (1998). Int Immunol. 10(4):517-26)
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2bκ in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-CD70, clone FR70, Cat. No. MABF3230, is a rat monoclonal antibody that detects mouse CD70 and is tested for use in Immunoprecipitation, Western Blotting, Flow Cytometry, and Inhibition Assay.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunoprecipitation
      • Inhibition
      Application NotesTested Applications

      Inhibition Assay: A representative lot inhibited binding of a murine CD27 Fc fusion protein to mouse CD70 in Inhibition Assays (Oshima, H., et al. (1998). Int Immunol. 10(4):517-26).

      Flow Cytometry Analysis: A 1:500 dilution from a representative lot detected CD70 in P815 cells overexpressing mouse CD70.

      Flow Cytometry Analysis: A representative lot detected CD70 in Flow Cytometry application (Oshima, H., et al. (1998). Int Immunol. 10(4):517-26).

      Western Blotting Analysis: A representative lot detected CD70 in Western Blotting application (Oshima, H., et al. (1998). Int Immunol. 10(4):517-26).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated CD70 in Immunoprecipitation application (Oshima, H., et al. (1998). Int Immunol. 10(4):517-26).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenA20 cells
      EpitopeExtracellular domain
      CloneFR70
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone FR70 is a rat monoclonal antibody that detects mouse CD70. It targets an epitope within the extracellular domain.
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cd70
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightTarget molecular weight 22 kDa observed; 21.92 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse splenocytes lysate.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected CD70 in mouse splenocytes lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3230-25UG 04065272290401

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