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MABD122 Anti-CD73 Antibody, clone AA60-E3-3

Detect CD73 using this mouse monoclonal antibody, Anti-CD73 Antibody, clone AA60-E3-3 validated for use in ICC, Flow Cytometry, western blotting & IP.

Anti-CD73 Antibody, clone AA60-E3-3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Mesenchymal Stem Cells Gene Symbol: NT5E, NT5, NTE UniProt Number: P21589
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MABD122
100 µg  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, FC, WB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABD122
      ReplacesMABC668
      DescriptionAnti-CD73 Antibody, clone AA60-E3-3
      Alternate Names
      • 5'-nucleotidase
      • 5'-NT
      • ecto-5'-nucleotidase
      Background InformationCD73, also known as Ecto-5'-nucleotidase, catalyzes the conversion of purine 5' mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation, being expressed on subsets of B-cells and T-cells. CD73 is also a marker of undifferentiated mesenchymal stem cells and is often used to identified stem cell populations.
      References
      Product Information
      FormatPurified
      Control
      • Human Adipose Mesenchymal Stem Cells
      PresentationPurified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect CD73 using this mouse monoclonal antibody, Anti-CD73 Antibody, clone AA60-E3-3 validated for use in ICC, Flow Cytometry, western blotting & IP.
      Key Applications
      • Immunocytochemistry
      • Flow Cytometry
      • Western Blotting
      • Immunoprecipitation
      Application NotesThis antibody has been shown to work in immunocytochemical applications on human adipose mesenchymal stem cells. This antibody has been shown to work in western blot applications on HMEC-1 cells by an independant lab.This antibody has been shown to work in immunoprecipitation applications on HMEC-1 cells by an independant lab.
      Biological Information
      ImmunogenWhole Mouse Dermal Lymphatic Endothelial Cells (DLEC)
      CloneAA60-E3-3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with human. Predicted to work in mouse based on immunogen design.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NT5E
      • NT5
      • NTE
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight63 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry on human adipose mesenchymal stem cells.
      Flow Cytometry Analysis: 1 µg/mL of this antibody detected CD73 on 1X10^6 human adipose mesenchymal stem cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABD122 04053252862946

      Documentation

      Anti-CD73 Antibody, clone AA60-E3-3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CD73 Antibody, clone AA60-E3-3 Certificates of Analysis

      TitleLot Number
      Anti-CD73, clone AA60-E3-3 - 3228347 3228347
      Anti-CD73, clone AA60-E3-3 - 3276090 3276090
      Anti-CD73, clone AA60-E3-3 - 3282010 3282010
      Anti-CD73, clone AA60-E3-3 - 3318619 3318619
      Anti-CD73, clone AA60-E3-3 - 3498496 3498496
      Anti-CD73, clone AA60-E3-3 - 4095351 4095351
      Anti-CD73, clone AA60-E3-3 - 4103082 4103082
      Anti-CD73, clone AA60-E3-3 - Q2153163 Q2153163
      Anti-CD73, clone AA60-E3-3 -2569204 2569204
      Anti-CD73, clone AA60-E3-3 -2648203 2648203
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